Structural characteristics of lower respiratory tract microflora in patients with pneumoconiosis.
10.3760/cma.j.cn121094-20220328-00154
- Author:
Jin E DAI
1
;
Ji Ying ZHANG
1
;
Di QIAO
2
;
Sheng Tao ZHAO
1
;
Xin ZHANG
1
;
Shao Ying LI
1
Author Information
1. Department of Respiratory Medicine, 920th Hospital of Chinese People's Liberation Army Joint Logistic Support Force, Kunming 650032, China.
2. Kunming Tongren Hospital, Kunming 650228, China.
- Publication Type:Journal Article
- Keywords:
16SrRNA gene sequencing;
Bronchoalveolar lavage fluid;
Pneumoconiosis;
Pulmonary flora
- MeSH:
Humans;
Cross-Sectional Studies;
Prospective Studies;
Pneumoconiosis;
Bacteria/genetics*;
Dust;
Respiratory System
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2023;41(4):247-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the composition of bacteria in lower respiratory tract of patients with pneumoconiosis and dust exposure, and to compare and analyze the difference and correlation between them. Methods: From May 2020 to January 2021, a prospective multicenter cross-sectional study was conducted to select patients with pneumoconiosis who underwent bronchoalveolar lavage treatment at the Respiratory and Critical Care Medical Department of the 920th Hospital of the Joint Support Force and the Respiratory Department of Tongren Hospital in Kunming, as well as the population of dust recipients. A total of 24 patients with pneumoconiosis (pneumoconiosis group) were included, and 16 dust exposed individuals (dust exposed group) were used as controls. Two groups of patients' alveolar lavage fluid were collected. The 16SrRNA gene V3-V4 sequencing technology and bioinformatics analysis platform were used to measure and analyze the differences in microbial structure composition and associations between bacterial communities. Results: Compared with the dust exposed group, the top 5 bacterial phyla in the alveolar lavage fluid level of patients with pneumoconiosis were the same, followed by Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Actinobacteria. Compared with the dust exposure group, the pneumoconiosis group patients belong to the top 5 genera of horizontal flora abundance, which are different. The dust exposure group is respectively: Pseudomonas, Proctor, Streptococcus, Achromobacter, and Neisseria. The pneumoconiosis group is respectively: Pseudomonas, Achromobacter, Streptococcus, Ralstonia, and Proctor. The Alpha diversity analysis results showed that compared with the dust exposed group, the level of bacterial diversity in the pneumoconiosis group was difference (P<0.05), and there was no statistically significant difference in bacterial evenness (P>0.05) ; Beta diversity showed differences in microbial community structure between the two groups (P<0.05 ). Single factor microbial association network analysis showed that there was a high correlation between Firmicutes and Bacteroidetes in the pneumoconiosis and dust exposed groups and other species, showing a positive correlation; The correlation between Proteobacteria and other species is high, showing a negative correlation. Conclusion: The structure and relative abundance of bacteria in lower respiratory tract were different between patients with pneumoconiosis and dust exposure, and the diversity of bacteria in lower respiratory tract increased in patients with pneumoconiosis, which may be related to disease status.
