Rapid determination of acetaminophen in plasma by LC-MS/MS.
10.3760/cma.j.cn121094-20220330-00163
- Author:
Yan YU
1
;
Hui Ling LI
1
;
Jing MA
1
;
Bo ZHOU
1
;
Fang DONG
1
Author Information
1. Department of Occupational Disease and Toxicology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
- Publication Type:Journal Article
- Keywords:
Acetaminophen;
Blood drug concentration;
Drug poisoning;
LC-MS/MS;
Plasma
- MeSH:
Humans;
Chromatography, Liquid/methods*;
Acetaminophen;
Tandem Mass Spectrometry/methods*;
Methanol;
Chromatography, High Pressure Liquid/methods*
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2023;41(5):364-366
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the rapid determination of acetaminophen (APAP) in human plasma by LC-MS/MS. Methods: The plasma samples were extracted by methanol and acetonitrile (1: 1) and purified directly. C(18) column was used for sample separation. The mobile phase were methanol (5 mmol/L ammonium acetate) and water (5 mmol/L ammonium acetate). Samples were analyzed by LC MS/MS with the electrospray ionization multi reaction monitoring (MRM) mode. Results: The calibration curves of APAP was linear in the concentration range of 0~10 mg/L, the correlation coefficient (r) was greater than 0.999 0. The relative standard deviation within and between batches was less than 10%. The recovery rate were 96.81%~101.7%. The detection limit of the method was 0.1 μg/L and the lower limit of quantification was 0.3 μg/L. Conclusion: This method has strong specificity, high sensitivity and reliable determination results. It is suitable for the rapid analysis of clinical plasma samples.
