Population structure of food-borne Staphylococcus aureus in China.
10.3760/cma.j.cn112338-20221206-01043
- VernacularTitle:我国食品来源金黄色葡萄球菌种群结构分析
- Author:
Ya Hui GUO
1
;
Zi Long HE
2
;
Qing Long JI
3
;
Hai Jian ZHOU
4
;
Fan Liang MENG
4
;
Xiao Feng HU
5
;
Xiao Yue WEI
4
;
Jun Cai MA
6
;
Yu Hua YANG
4
;
Wei ZHAO
7
;
Li Jin LONG
4
;
Xin WANG
8
;
Jia Ming FAN
4
;
Xiao Jie YU
9
;
Jian Zhong ZHANG
4
;
De HUA
9
;
Xiao Mei YAN
4
;
Hai Bin WANG
10
Author Information
1. Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. Beijing Advanced Innovation Center for Big Data-based Precision Medicine, Interdisciplinary Innovation Institute of Medicine and Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China.
3. Chinese Academy of Inspection and Quarantine, Beijing 100020, China.
4. State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
5. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100032, China.
6. Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
7. Institute of Microbiology, Jilin Provincial Center for Disease Control and Prevention, Changchun 130051, China.
8. College of Food Science and Engineering, Northwest Agriculture & Forestry University, Xi'an 712100, China.
9. Hainan Center for Disease Control and Prevention, Haikou 570203, China.
10. Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China Chaoyang District Center for Disease Control and Prevention, Beijing 100020, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Staphylococcus aureus/genetics*;
Methicillin-Resistant Staphylococcus aureus/genetics*;
Multilocus Sequence Typing;
Phylogeny;
Staphylococcal Infections/epidemiology*;
China/epidemiology*
- From:
Chinese Journal of Epidemiology
2023;44(6):982-989
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To understand the population structure of food-borne Staphylococcus (S.) aureus in China. Methods: Whole genome sequencing was used to analyze 763 food-borne S. aureus strains from 16 provinces in China from 2006 to 2020. Multilocus sequence typing (MLST), staphylococcal protein A gene (spa) typing, and staphylococcal chromosome cassettemec (SCCmec) typing were conducted, and minimum spanning tree based on ST types (STs) was constructed by BioNumerics 7.5 software. Thirty-one S. aureus strains isolated from imported food products were also included in constructing the genome phylogenetic tree. Results: A total of 90 STs (20 novel types) and 160 spa types were detected in the 763 S. aureus isolates. The 72 STs (72/90, 80.0%) were related to 22 clone complexes. The predominant clone complexes were CC7, CC1, CC5, CC398, CC188, CC59, CC6, CC88, CC15, and CC25, accounting for 82.44% (629/763) of the total. The STs and spa types in the predominant clone complexes changed over the years. The methicillin-resistant S. aureus (MRSA) detection rate was 7.60%, and 7 SCCmec types were identified. The ST59-t437-Ⅳa (17.24%, 10/58), ST239-t030-Ⅲ (12.07%, 7/58), ST59-t437-Ⅴb (8.62%, 5/58), ST338-t437-Ⅴb (6.90%, 4/58) and ST338-t441-Ⅴb (6.90%, 4/58) were the main types in MRSA strains. The genome phylogenetic tree had two clades, and the strains with the same CC, ST, and spa types clustered together. All CC7 methicillin sensitive S. aureus strains were included in Clade1, while 21 clone complexes and all MRSA strains were in Clade2. The MRSA strains clustered according to the SCCmec and STs. The strains from imported food products in CC398, CC7, CC30, CC12, and CC188 had far distances from Chinese strains in the tree. Conclusions: In this study, the predominant clone complexes of food-borne strains were CC7, CC1, CC5, CC398, CC188, CC59, CC6, CC88, CC15, and CC25, which overlapped with the previously reported clone complexes of hospital and community-associated strains in China, suggesting that close attention needs to be paid to food, a vehicle of pathogen transmission in community and food poisoning.
