Study of the urban-impact on microbial communities and their virulence factors and antibiotic resistance genomes in the Nandu River, Haikou.
10.3760/cma.j.cn112338-20221229-01090
- VernacularTitle:城市对海口市南渡江的微生物群落及其毒力因子与抗生素耐药基因组的影响
- Author:
Yu Feng FAN
1
;
Zhen Peng LI
1
;
Xiao Jie YU
2
;
Zhe LI
1
;
Hai Jian ZHOU
1
;
Ya Lin ZHANG
2
;
Xiao Ting GAN
2
;
De HUA
2
;
Xin LU
1
;
Biao KAN
3
Author Information
1. Department of Diarrheal Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. Inspection and Testing Institute, Hainan Center for Disease Control and Prevention, Haikou 570203, China.
3. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Rivers;
Virulence Factors/genetics*;
RNA, Ribosomal, 16S/genetics*;
Microbiota/genetics*;
Anti-Bacterial Agents;
Drug Resistance, Microbial/genetics*
- From:
Chinese Journal of Epidemiology
2023;44(6):974-981
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the changes in bacterial community structure, antibiotic resistance genome, and pathogen virulence genome in river water before and after the river flowing through Haikou City and their transmission and dispersal patterns and to reveal anthropogenic disturbance's effects on microorganisms and resistance genes in the aquatic environment. Methods: The Nandu River was divided into three study areas: the front, middle and rear sections from the upstream before it flowed through Haikou City to the estuary. Three sampling sites were selected in each area, and six copies of the sample were collected in parallel at each site and mixed for 3 L per sample. Microbial community structure, antibiotic resistance, virulence factors, and mobile genetic elements were analyzed through bioinformatic data obtained by metagenomic sequencing and full-length sequencing of 16S rRNA genes. Variations in the distribution of bacterial communities between samples and correlation of transmission patterns were analyzed by principal co-ordinates analysis, procrustes analysis, and Mantel test. Results: As the river flowed through Haikou City, microbes' alpha diversity gradually decreased. Among them, Proteobacteria dominates in the bacterial community in the front, middle, and rear sections, and the relative abundance of Proteobacteria in the middle and rear sections was higher than that in the front segment. The diversity and abundance of antibiotic resistance genes, virulence factors, and mobile genetic elements were all at low levels in the front section and all increased significantly after flow through Haikou City. At the same time, horizontal transmission mediated by mobile genetic elements played a more significant role in the spread of antibiotic-resistance genes and virulence factors. Conclusions: Urbanization significantly impacts river bacteria and the resistance genes, virulence factors, and mobile genetic elements they carry. The Nandu River in Haikou flows through the city, receiving antibiotic-resistant and pathogen-associated bacteria excreted by the population. In contrast, antibiotic-resistant genes and virulence factors are enriched in bacteria, which indicates a threat to environmental health and public health. Comparison of river microbiomes and antibiotic resistance genomes before and after flow through cities is a valuable early warning indicator for monitoring the spread of antibiotic resistance.
