Genetic characterization of varicella-zoster virus in people aged 20 years and under in Yichang City of Hubei Province, 2019-2020.
10.3760/cma.j.cn112338-20221027-00915
- Author:
Mei Ying YOU
1
;
Miao Miao WANG
1
;
Hong GUO
2
;
Tian Qi WANG
3
;
Xu Dong LI
1
;
Song Tao XU
2
;
Yue Hua HU
1
;
Da Ping YIN
4
Author Information
1. Office of Epidemiology, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. National Health Commision Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
3. Office of Epidemiology, Chinese Center for Disease Control and Prevention, Beijing 102206, China Data Resources and Statistics Department, Beijing Municipal Health Big Data and Policy Research Center, Beijing 100034, China.
4. Hainan Center for Disease Control and Prevention, Haikou 570203, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Child;
Adolescent;
Young Adult;
Adult;
Herpesvirus 3, Human/genetics*;
Phylogeny;
Herpes Zoster/epidemiology*;
Polymorphism, Single Nucleotide;
Real-Time Polymerase Chain Reaction;
Nucleotides
- From:
Chinese Journal of Epidemiology
2023;44(4):607-610
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the genetic characteristics of varicella-zoster virus (VZV) in people aged 20 years and under in Yichang City of Hubei Province from 2019 to 2020. Methods: Based on the Yichang Health Big Data Platform, we investigated cases 20 and under clinically diagnosed as herpes zoster in three hospitals from March 2019 to September 2020. Collecting vesicle fluid and throat swab samples of the cases and completing questionnaires to obtain basic information. Real-time fluorescent quantitative PCR was used for positive identification of the virus. PCR amplification of VZV's open reading frame (ORF) and sequencing of the products to determine the VZV genotype. Analyze mutations at some specific single nucleotide polymorphism (SNP) sites. Results: Among 46 cases of herpes zoster, the male to female ratio was 1.3∶1 (26∶20) and the age ranged from 7 to 20 years old. Fifteen cases had been vaccinated against varicella, including 13 and 2 cases of 1 and 2 doses, respectively. VZV strains were detected in 34 samples (73.91%), all belonging to Clade 2. Phylogenetic tree analysis of the nucleotide of ORF22 showed, compared with Clade 2 referenced strains, the sequence matching degree of nucleotide for all 34 samples was 99.0% to 100.0%. Conclusion: The main VZV strain causing herpes zoster in people aged 20 years and under in Yichang from 2019 to 2020 was Clade 2.
