Application of mixture analysis methods in association between metals mixture exposure and DNA oxidative damage.
10.3760/cma.j.cn112150-20221031-01046
- Author:
Yan Hua WANG
1
;
Hui Ge YUAN
1
;
Li Ya ZHANG
1
;
Yang LIN
1
;
Ting WANG
1
;
Huan XU
2
;
Xing ZHAO
2
;
Hua Wei DUAN
1
Author Information
1. National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
2. West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Adult;
Nickel/toxicity*;
Selenium;
Bayes Theorem;
Metals/toxicity*;
8-Hydroxy-2'-Deoxyguanosine;
Oxidative Stress/physiology*;
Zinc;
DNA Damage
- From:
Chinese Journal of Preventive Medicine
2023;57(7):1026-1031
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To study the association between metals mixture exposure and DNA oxidative damage using mixture analysis methods, and to explore the most significant exposure factors that cause DNA oxidative damage. Methods: Workers from steel enterprises were recruited in Shandong Province. Urinary metals were measured by using the inductively coupled plasma mass spectrometry method. The level of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was determined by using the ultra-high performance liquid chromatography-mass spectrometry method. Bayesian kernel machine regression (BKMR), elastic net regression and quantile g-computation regression were used to analyze the association between urinary metals and urinary 8-OHdG. Results: A total of 768 subjects aged (36.15±7.40) years old were included in the study. BKMR, elastic net regression and quantile g-computation all revealed an overall positive association between the mixture concentration and increased urinary 8-OHdG. The quantile g-computation results showed that with a 25% increase in metal mixtures, the urinary 8-OHdG level increased by 77.60%. The elastic net regression showed that with a 25% increase in exposure risk score, the urinary 8-OHdG level increased by 26%. The BKMR summarized the contribution of individual exposures to the response, and selenium, zinc, and nickel were significant contributors to the urinary 8-OHdG elevation. Conclusion: Exposure to mixed metals causes elevated levels of DNA oxidative damage, and selenium, zinc, and nickel are significant exposure factors.
