Pathogenic characterization of Klebsiella pneumoniae resistant to carbapenems and polymyxin.
10.3760/cma.j.cn112150-20230111-00023
- Author:
Xi CHEN
1
;
Zhao Hui SUN
2
;
Zhi Hui JIANG
3
;
Yi Xue WU
4
;
Zi Jing ZHU
4
;
Li Dan CHEN
2
Author Information
1. Graduate School, Guangzhou University of Chinese Medicine, Guangzhou 510006, China Department of Laboratory Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China.
2. Department of Laboratory Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, China.
3. Department of Pharmacy, General Hospital of Southern Theater Command, Guangzhou 510010, China.
4. Department of Laboratory Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Carbapenems/pharmacology*;
Anti-Bacterial Agents/therapeutic use*;
Klebsiella pneumoniae/genetics*;
Polymyxins/pharmacology*;
beta-Lactamases;
Phylogeny;
Retrospective Studies;
Multilocus Sequence Typing;
Microbial Sensitivity Tests
- From:
Chinese Journal of Preventive Medicine
2023;57(6):877-884
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Analysis and investigation of pathogenic characteristics of polymyxin-and carbapenem-resistant Klebsiella pneumoniae (PR-CRKP). Methods: A total of 23 PR-CRKP strains isolated from clinical specimens from the General Hospital of Southern Theater Command from March 2019 to July 2021 were retrospectively collected, Whole-genome sequencing was performed on 23 PR-CRKP strains, resistance genes were identified by comparison of the CARD and the ResFinder database, high-resolution typing of PR-CRKP strains was analyzed by core genomic multilocus sequencing (cgMLST) and single nucleotide polymorphism (SNP); polymyxin resistance genes were determined by PCR and sequencing. Results: All PR-CRKP strains were KPC-2 producing ST11 types. cgMLST results showed that the evolutionary distance between the PR-CRKP strains and Klebsiella pneumoniae in mainland China was 66.44 on average, which is more closely related than foreign strains; the 23 PR-CRKP strains were divided into 3 main subclusters based on SNP phylogenetic trees, with some aggregation among Clade 2-1 in the isolation department and date. The two-component negative regulatory gene mgrB has seven mutation types including point mutations, different insertion fragments and different insertion positions. Conclusion: The close affinity of PR-CRKP strains indicate the possibility of nosocomial clonal transmission and the need to strengthen surveillance of PR-CRKP strains to prevent epidemic transmission of PR-CRKP.
