Developmental effects of TCIPP and TnBP on zebrafish (Danio rerio) embryos.
10.3760/cma.j.cn112150-20230218-00124
- VernacularTitle:有机磷阻燃剂TCIPP和TnBP对斑马鱼胚胎发育影响的研究
- Author:
Shu Yi ZHANG
1
;
Shao Ping ZHANG
2
;
Zi Jin SHAO
3
;
Yuan Zheng FU
3
;
Wen GU
2
;
Hong ZHI
2
;
Jian KONG
2
;
Fu Chang DENG
2
;
Wen Yan YAN
1
;
Juan LIU
2
;
Chao WANG
2
;
Song TANG
1
Author Information
1. Center for Global Health, School of Public Health/Nanjing Medical University, Nanjing 211166, China China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health/Chinese Center for Disease Control and Prevention, Beijing 100021, China.
2. China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health/Chinese Center for Disease Control and Prevention, Beijing 100021, China.
3. China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health/Chinese Center for Disease Control and Prevention, Beijing 100021, China Department of Toxicology, School of Public Health, China Medical University, Shenyang 110122, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Zebrafish/metabolism*;
Embryo, Nonmammalian/metabolism*;
Transcriptome;
Oxidative Stress;
Water Pollutants, Chemical/metabolism*
- From:
Chinese Journal of Preventive Medicine
2023;57(5):693-700
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the toxicity of tris (2-chloropropyl) phosphate (TCIPP) and tributyl phosphate (TnBP) on the growth and development of zebrafish embryos, as well as to explore the underlying mechanisms at the transcriptional level. Methods: With zebrafish as a model, two hpf zebrafish embryos were exposed to TCIPP and TnBP (0.1, 1, 10, 100, 500, and 1 000 μmol/L) using the semi-static method, and their rates of lethality and hatchability were determined. The transcriptome changes of 120 hpf juvenile zebrafish exposed to environmentally relevant concentrations of 0.1 and 1 μmol/L were measured. Results: The 50% lethal concentrations (LC50) of TCIPP and TnBP for zebrafish embryos were 155.30 and 27.62 μmol/L (96 hpf), 156.5 and 26.05 μmol/L (120 hpf), respectively. The 72 hpf hatching rates of TCIPP (100 μmol/L) and TnBP (10 μmol/L) were (23.33±7.72)% and (91.67±2.97)%, which were significantly decreased compared with the control group (P<0.05). Transcriptome analysis showed that TnBP had more differential genes (DEGs) than TCIPP, with a dose-response relationship. These DEGs were enriched in 32 pathways in total, including those involved in oxidative stress, energy metabolism, lipid metabolism, and nuclear receptor-related pathways, using the IPA pathway analysis. Among them, three enriched pathways overlapped between TCIPP and TnBP, including TR/RXR activation and CAR/RXR activation. Additionally, DEGs were also mapped onto pathways of LXR/RXR activation and oxidative stress for TnBP exposure only. Conclusion: Both TCIPP and TnBP have growth and developmental toxicities in zebrafish embryos, with distinct biomolecular mechanisms, and TnBP has a stronger effect than TCIPP.
