Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method.

Xiang Sheng; Min LI; Ya Li Wang; Yu Ling Chen; Yuan Lin; Zhen Min Zhao; Ting Zhi Que

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Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method.

Author: Xiang SHENG ¹ ; Min LI ² ; Ya Li WANG ² ; Yu Ling CHEN ² ; Yuan LIN ² ; Zhen Min ZHAO ² ; Ting Zhi QUE ²
Author Information

1. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.
2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, PRC, Shanghai 200063, China.
Publication Type:Journal Article
Keywords: Chelex-100 method; MPure-12 automatic nucleic acid purification; automation, laboratory; extraction; forensic genetics
MeSH: Alleles; Blood Stains; Chelating Agents; DNA/isolation & purification*; DNA Fingerprinting; Forensic Medicine/methods*; Genotype; Humans; Male; Polymerase Chain Reaction/methods*; Polystyrenes; Polyvinyls; Resins, Synthetic; Saliva; Semen/chemistry*
From: Journal of Forensic Medicine 2017;33(2):168-170
CountryChina
Language:Chinese
Abstract:
OBJECTIVESTo explore the forensic application value of MPure-12 automatic nucleic acid purification （MPure-12 Method） for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents.
METHODSNine types of biological samples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR-profiles.
RESULTSThe samples such as hair root, chutty, butt, muscular tissue, saliva stain, bloodstain and semen stain were typed successfully by MPure-12 method. Partial alleles were lacked in the samples of saliva, and the genotyping of contact swabs was unsatisfactory. Additional, all of the bloodstains （20 μL, 15 μL, 10 μL, 5 μL, 1 μL） showed good typing results using Chelex-100 method. But the loss of alleles occurred in 1 μL blood volume by MPure-12 method.
CONCLUSIONSMPure-12 method is suitable for DNA extraction of a certain concentration blood samples．Chelex-100 method may be better for the extraction of trace blood samples．This instrument used in nucleic acid extraction has the advantages of simplicity of operator, rapidity, high extraction efficiency, high rate of reportable STR-profiles and lower man-made pollution.