Determination of Three Types of New Psychoactive Tryptamines in Blood by QuEChERS Combined with UPLC-MS/MS.
10.12116/j.issn.1004-5619.2021.310205
- Author:
Wei HOU
1
;
Yan Yan WANG
1
;
Ying ZHANG
1
;
Lei Ping ZHANG
2
;
Guo Bin XIN
1
;
Shi Yang QIN
1
;
Ji Fen WANG
3
Author Information
1. Key Laboratory of Forensic Toxicology, Ministry of Public Security, Beijing Public Security Bureau, Beijing 100192, China.
2. Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China.
3. School of Investigation, People' s Public Security University of China, Beijing 100038, China.
- Publication Type:Journal Article
- Keywords:
forensic medicine;
toxicological analysis;
new psychoactive substance;
tryptamines;
QuEchERS;
ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）;
5-MeO-DALT;
5-MeO-MiPT;
5-MeO-DiPT;
blood
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Humans;
Limit of Detection;
Tandem Mass Spectrometry;
Tryptamines
- From:
Journal of Forensic Medicine
2021;37(4):516-523
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method combining QuEChERS and ultra-high liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for rapid screening and testing of three types of new psychoactive tryptamines in human blood： 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT. Methods The effects of the type of extractant, the type and dosage of salting-out agent, and the dosage of adsorbent on the test results of the three tryptamines were investigated. Blood samples were processed by QuEChERS method and then determined by UPLC-MS/MS. Results The linear relationships of 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT in human blood were good in the range of 0.5-100, 0.5-100 and 0.2-100 ng/mL, respectively, with their coefficients higher than 0.99. The limits of detection （LODs） were 0.1-0.2 ng/mg. The recoveries ranged from 84.86% to 94.57%. Intra-day and inter-day precisions were good. Conclusion The method is simple, rapid, easy to operate and has a high recovery. It is suitable for the qualitative and quantitative study of tryptamines in blood and can provide the reference for public security organs to deal with related cases.
