Metabolism of New Psychoactive Substances 4F-MDMB-BUTINACA in Zebrafish.
10.12116/j.issn.1004-5619.2021.310401
- Author:
Lin Na YUE
1
;
Ping XIANG
1
;
Fen Yun SONG
2
;
Bao Hua SHEN
1
;
Hui YAN
1
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
2. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
forensic medicine;
toxicological analysis;
synthetic cannabinoids;
metabolic pathway;
liquid chromatography （LC）;
high resolution mass spectrometry （HRMS）;
4F-MDMB-BUTINACA;
zebrafish
- MeSH:
Animals;
Cannabinoids;
Chromatography, Liquid;
Illicit Drugs;
Microsomes, Liver/chemistry*;
Zebrafish
- From:
Journal of Forensic Medicine
2021;37(4):493-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the metabolic transformation pathways of 4F-MDMB-BUTINACA in vivo by establishing zebrafish models. Methods Six adult zebrafish were randomly divided into blank control group and experimental group, with three fish in each group. After the zebrafish in the experimental group were exposed to 1 μg/mL 4F-MDMB-BUTINACA for 24 h, they were transferred to clean water and cleaned three times, then pretreated for instrumental analysis. The zebrafish in blank control group were not exposed to 4F-MDMB-BUTINACA. Mass spectrometry and structural analysis of 4F-MDMB-BUTINACA and its metabolites were conducted by liquid chromatography-high resolution mass spectrometry and Mass Frontier software. Results A total of twenty-six metabolites of 4F-MDMB-BUTINACA were identified in zebrafish, including eighteen phase Ⅰ metabolites and eight phase Ⅱ metabolites. The main metabolic pathways of phase Ⅰ metabolites of 4F-MDMB-BUTINACA in zebrafish were ester hydrolysis, N-dealkylation, oxidative defluorination and hydroxylation, while the main metabolic pathway of phase Ⅱ metabolites was glucuronidation. Conclusion Metabolite Md24 （ester hydrolysis） and Md25 （ester hydrolysis combined with dehydrogenation） would be recommended to be potentially good biomarkers for abuse of 4F-MDMB-BUTINACA.
