Determination of Azide Ions in Blood by Pentafluorobenzyl Derivation Followed by GC-MS.
10.12116/j.issn.1004-5619.2020.300304
- Author:
Mao Sheng LI
1
;
Shui Qing ZHENG
1
;
Zhen Hai SHENG
1
;
Si Yang HE
1
;
Qian Ya DENG
1
;
Chen LIANG
1
;
Zhong Ping WU
1
;
Fang Qi CAO
2
;
Meng DU
3
Author Information
1. Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science of Shanghai Public Security Bureau, Shanghai 200083, China.
2. Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Research Institute of Criminal Science and Technology, Shanghai 200083, China.
3. Institute of Criminal Science, Jiading Branch of Shanghai Public Security Bureau, Shanghai 201800, China.
- Publication Type:Journal Article
- Keywords:
forensic toxicology;
gas chromatography-mass spectrometry;
pentafluorobenzyl derivatization;
azide ion;
cyanide ion
- MeSH:
Azides;
Gas Chromatography-Mass Spectrometry;
Humans;
Ions
- From:
Journal of Forensic Medicine
2021;37(3):378-381
- CountryChina
- Language:English
-
Abstract:
Objective To establish a method for determination of the azide ions in blood by gas chromatography-mass spectrometry （GC-MS） following pentafluorobenzyl derivatization. Methods A blood sample of 0.2 mL was placed into a 10 mL glass test tube, and the internal standard sodium cyanide, derivatization reagent pentafluorobenzyl bromide and catalyst tetradecyl benzyl dimethyl ammonium chloride were added in turn. After vortex mixing, the mixture was heated with low-power microwave for 3 min. After centrifugation, the organic phase was taken for GC-MS analysis. Results The azide ions in blood had a good linear relationship in the mass concentration range of 0.5 to 20 μg/mL. The lowest detection limit was 0.25 μg/mL and the relative recovery was 91.36%-94.58%. The method was successfully applied to a case of death from sodium azide poisoning. The mass concentration of azide ions in the blood of the dead was 11.11 μg/mL. Conclusion The method developed in this paper has strong specificity and is easy to operate, which is suitable for the rapid detection of azide ions in blood.
