Application of Automatic Nucleic Acid Extractor Combined with Vacuum Concentrator in Forensic Science.
10.12116/j.issn.1004-5619.2020.500604
- Author:
Shu Xia LI
1
;
Jin Jian SHEN
1
;
Pei Jun CAO
1
;
Zhi Chao LI
2
Author Information
1. Pinghu Public Security Bureau, Pinghu 314200, Zhejiang Province, China.
2. Fengxian Branch of Shanghai Public Security Bureau, Shanghai 201499, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
DNA extraction;
trace DNA;
STR typing;
inhibitor;
automatic nucleic acid extractor;
vacuum concentrator
- MeSH:
DNA/genetics*;
DNA Fingerprinting;
Humans;
Microsatellite Repeats;
Nucleic Acids;
Vacuum
- From:
Journal of Forensic Medicine
2021;37(2):220-224
- CountryChina
- Language:English
-
Abstract:
Objective To explore the application value of automatic nucleic acid extractor combined with vacuum concentrator in forensic DNA extraction. Methods Gradient samples of human peripheral venous blood were collected at 40, 80, 120, 160, 200, 240, 280 and 320 fold dilution. The samples of each gradient were treated with no inhibitor, black oil, rust, fruit acid, tin foil and indigo, respectively. The automatic nucleic acid extractor was used for DNA purification and extraction of the above samples. The extracted DNA eluent （6 μL） was taken for amplification directly, and the rest was concentrated by vacuum concentrator. DNA was amplified and examined using the Investigator 26plex QS kit before and after concentration. Results Only gradient samples treated with fruit acid obtained complete STR typing results at 40 fold dilution. The other 5 methods obtained complete STR typing results at 40-160 fold dilution. The results of STR typing after DNA concentration showed that the average peak height and detection rates of gene loci both increased to a certain extent, but the effect was not obvious. Conclusion The automatic nucleic acid extractor has an efficient inhibitor removal ability and high extracting efficiency of DNA. The vacuum concentrator can concentrate DNA samples to a certain extent. Combining the automatic nucleic acid extractor with the vacuum concentrator can improve the examination success rate of forensic materials.
