DNA Methylation Differences in Peripheral Blood of Patients with Anaphylaxis.
10.12116/j.issn.1004-5619.2019.490414
- Author:
Ming Zhe LIU
1
;
Hua Lin GUO
1
;
Yan FENG
2
;
Jian Guo LI
3
;
Peng Fei LI
1
;
Cai Rong GAO
1
;
Xiang Jie GUO
1
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, Shanxi Province, China.
2. First Hospital of Shanxi Medical University, Taiyuan 030000, China.
3. China Institute for Radiation Protection, Taiyuan 030000, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
anaphylactic response;
DNA methylation;
DNA chip;
drug anaphylaxis
- MeSH:
Anaphylaxis/genetics*;
DNA Methylation;
Forensic Medicine;
Humans
- From:
Journal of Forensic Medicine
2021;37(2):211-214
- CountryChina
- Language:English
-
Abstract:
Objective To study the DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and to provide a new research direction and basis for the forensic diagnosis of shock caused by drug hypersensitiveness. Methods Methylation microarray was used to detect DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and normal subjects. Sequencing data and chip data were analyzed for differences in DNA methylation using R language methylkit, ChAMP package. Random forest algorithm was used to evaluate the importance of the DNA methylation differential sites. Results Differential sites of DNA methylation highly associated with anaphylaxis caused by cephalosporin drugs were obtained at loci such as ETS1, PRR23B and GNAS. Conclusion Cephalosporin allergy is associated with DNA methylation, and DNA methylation may be a new strategy for forensic identification of anaphylactic shock and death.
