Supramolecular Solvent Extraction-Gas Chromatography-Tandem Mass Spectrometry for Detection of Benzodiazepines in Urines.
10.12116/j.issn.1004-5619.2020.300402
- Author:
Jin Lei LIU
1
;
Jie GU
2
;
Li Qin CHEN
2
;
Wurita AMIN
2
Author Information
1. Graduate School of Inner Mongolia Medical University, Hohhot 010059, China.
2. Department of Forensic Medicine, Inner Mongolia Medical University, Hohhot 010110, China.
- Publication Type:Journal Article
- Keywords:
forensic toxicology;
gas chromatography-tandem mass spectrometer;
benzodiazepines;
supramolecular solvent;
urine
- MeSH:
Benzodiazepines;
Chromatography, Liquid;
Gas Chromatography-Mass Spectrometry;
Humans;
Solvents;
Tandem Mass Spectrometry
- From:
Journal of Forensic Medicine
2021;37(1):26-32
- CountryChina
- Language:English
-
Abstract:
Objective To establish a method using supramolecular solvent and gas chromatography-tandem mass spectrometry （GC-MS/MS） to analyze 9 benzodiazepines in urines. Methods Urine samples containing 9 benzodiazepines reference substance were subjected to liquid-liquid extractions with supramolecular solvent, which consisted of tetrahydrofuran and 1-hexanol. The solvent layer was evaporated to dryness by stream of nitrogen. The residue was reconstituted with methanol, and GC-MS/MS analysis was performed on it. The way of data collection was multiple reaction monitoring （MRM） mode; internal standard method was employed for quantification. Results In urine samples, when the range of mass concentration was 1-100 ng/mL for diazepam, midazolam, flunitrazepam and clozapine, 5-100 ng/mL for lorazepam and alprazolam, 2-100 ng/mL for nitrazepam and clonazepam, and 0.2-100 ng/mL for estazolam, respectively, good linearities were obtained, correlation coefficients were 0.999 1-0.999 9, the lower limits of the quantifications ranged from 0.2 to 5 ng/mL, the extraction recovery rates were 81.12%-99.52%. The intra-day precision ［relative standard deviation （RSD）］ and accuracy （bias） were lower than 9.86% and 9.51%, respectively; the inter-day precision （RSD） and accuracy （bias） were lower than 8.74% and 9.98%, respectively. Nine drugs in urine samples showed good stability at ambient temperature and -20 ℃ within 15 days. The mass concentrations of alprazolam in urine samples obtained from 8 volunteers who took alprazolam tablets orally within 8-72 h after ingestions ranged from 6.54 to 88.28 ng/mL. Conclusion The supramolecular solvent extraction GC-MS/MS method for analysis of 9 benzodiazepines in urines provided by this study is simple, fast, accurate and sensitive, which can provide technical support for monitoring of poisoning by benzodiazepines for clinical treatment and judicial identification.
