Mitochondrial DNA Heteroplasmy of Hair Shaft Using HID Ion GeneStudioTM S5 Sequencing System.
10.12116/j.issn.1004-5619.2019.590905
- Author:
Feng CHENG
1
;
Qing Xia ZHANG
2
;
Cheng Jian CHEN
2
;
Wan Ting LI
1
;
Jia Rong ZHANG
1
;
Geng Qian ZHANG
1
;
Jiang Wei YAN
1
Author Information
1. College of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
2. Forensic Central of Beijing Public Security Bureau, Beijing 100192, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
DNA, mitochondrial;
whole genome;
HID Ion GeneStudioTM S5 Sequencing System;
hair shaft
- MeSH:
DNA, Mitochondrial/genetics*;
Genome, Mitochondrial/genetics*;
Heteroplasmy;
High-Throughput Nucleotide Sequencing;
Humans;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2021;37(1):21-25
- CountryChina
- Language:English
-
Abstract:
Objective To study the heteroplasmy of the whole mitochondrial genome genotyping result of hair shaft samples using HID Ion GeneStudioTM S5 Sequencing System. Methods The buccal swabs and blood of 8 unrelated individuals, and hair shaft samples from different parts of the same individual were collected. Amplification of whole mitochondrial genome was performed using Precision ID mtDNA Whole Genome Panel. Analysis and detection of whole mitochondrial genome were carried out using the HID Ion GeneStudioTM S5 Sequencing System. Results The mitochondrial DNA sequences in temporal hair shaft samples from 2 individuals showed heteroplasmy, while whole mitochondrial genome genotyping results of buccal swabs, blood, and hair samples from the other 6 unrelated individuals were consistent. A total of 119 base variations were observed from the 8 unrelated individuals. The numbers of variable sites of the individuals were 29, 40, 38, 35, 13, 36, 40 and 35, respectively. Conclusion Sequence polymorphism can be fully understood using HID Ion GeneStudioTM S5 Sequencing system.
