Application of Two Kinds of Automatic Nucleic Acid Purifiers.
10.12116/j.issn.1004-5619.2020.06.009
- Author:
Wei CAI
1
;
Lei YU
2
Author Information
1. Dongyang Public Security Bureau, Dongyang 322100, Zhejiang Province, China.
2. Jinshan Branch of Shanghai Public Security Bureau, Shanghai 200540, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
DNA extraction;
trace DNA;
automatic nucleic acid purifier;
inhibitor
- MeSH:
DNA;
DNA Fingerprinting;
Nucleic Acids/genetics*;
Polymerase Chain Reaction
- From:
Journal of Forensic Medicine
2020;36(6):797-800
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of automatic nucleic acid purifiers QIAsymphony SP and QIAcube in the DNA extraction of samples of trace amount or mixed with inhibitors. Methods Different kinds of purification methods using QIAsymphony SP and QIAcube were applied to extract swabs which contained 30, 100, 150 and 300 cells and other samples which contained six types of inhibitors-heme, humic acid, lard, soil, rust and grease. PCR amplification and STR typing were performed on the extracted DNA templates to compare extracting efficiency and inhibitor removal ability of four different purification methods. Results Different purification methods showed similar extraction effects, 70.83%-100.00% of loci could be detected by amplification of DNA extracted from samples with 30, 100 and 300 cells, and the six types of inhibitors could be removed well. Conclusion The two automatic nucleic acid purifiers have a good inhibitor removal effect. For swabs with only 30 cells, after DNA extraction and amplification, the locus detection rate of samples can still be high, which can meet the requirements of local DNA laboratory work, and realize the standardization construction of the laboratory.
