Relationship between Electrical Conductivity and Chemical Content of Rat Skeletal Muscle Impregnating Solution and Postmortem Interval.
10.12116/j.issn.1004-5619.2019.05.011
- Author:
Zhe ZHENG
1
;
Xian-dun ZHAI
1
;
Zhi Yuan XIA
2
;
Yong Lin LI
1
;
Lin Lin ZHAO
1
;
Hui Li LIU
3
;
Yao Nan MO
1
Author Information
1. School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, Henan Province, China.
2. Institute of Evidence Law and Forensic Science, China University of Political Science and Law, Beijing 100088, China.
3. 3 Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
skeletal muscle;
electrical conductivity;
estimation of postmortem interval;
rats
- MeSH:
Animals;
Electric Conductivity;
Forensic Pathology;
Muscle, Skeletal;
Postmortem Changes;
Rats;
Rats, Sprague-Dawley;
Time Factors
- From:
Journal of Forensic Medicine
2019;35(5):572-575
- CountryChina
- Language:English
-
Abstract:
Objective To study the mechanism of change of the electrical conductivity （EC） of rat skeletal muscle impregnating solution that occurs with the change of postmortem interval （PMI）. Methods Healthy Sprague-Dawley rats were killed and kept at about 25 ℃. Skeletal muscles were extracted at different PMI--immediate （0 d）, 1 d, 2 d, 3 d, 4 d, 5 d, 6 d, and 7 d, then mixed with deionized water to make impregnating solution with a mass concentration of 0.1 g/mL. The solution's EC and nine common chemicals in it, such as potassium ion, calcium ion, and chloride ion, were determined. Results EC increased gradually with the extending of PMI （P=0.024） during the 7 days after the rats' death. The content of uric acid （P=0.032）, urea nitrogen （P=0.013） and phosphorus （P=0.022） also increased during the extension. However, the content of magnesium ions decreased with extending of PMI （P=0.047）. The correlation between potassium ion, sodium ion, chlorine ion, calcium ion, creatinine and PMI were weak （P>0.05）. Conclusion The molecular basis of skeletal muscle EC change in rats after their death is the changes of uric acid, urea nitrogen, inorganic phosphorus and other chemical components. Furthermore, combine use of various indicators can improve the accuracy of the EC method to infer PMI.
