Expression of Cx43 and Cx45 in Cardiomyocytes of an Overworked Rat Model.
10.12116/j.issn.1004-5619.2019.05.010
- Author:
Bo Fan YANG
1
;
Jing Zhuo SHI
1
;
Jing LI
1
;
Yu Peng PAN
1
;
Ning XIAO
1
;
Yan Geng YU
2
;
Fu ZHANG
2
;
Hui Jun WANG
1
;
Dong Ri LI
1
Author Information
1. School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
2. Key Laboratory of Forensic Pathology, Ministry of Public Security, Guangdong Provincial Public Security Department, Guangzhou 510050, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
heart injuries;
connexin 43;
connexin 45;
models, animal;
karoshi;
rats
- MeSH:
Animals;
Arrhythmias, Cardiac/metabolism*;
Connexin 43/metabolism*;
Connexins/metabolism*;
Heart Failure;
Myocardium;
Myocytes, Cardiac/metabolism*;
Rats
- From:
Journal of Forensic Medicine
2019;35(5):567-571
- CountryChina
- Language:English
-
Abstract:
Objective To study the effect of overwork stress response on the expression of connexin 43（Cx43） and connexin 45（Cx45） in cardiomyocytes and on cardiac function. Methods The experimental animals were divided into control group, overworked 1-month group and overworked 2-month group. A overworked rat model was established by forcing swimming of overworked group. The expressions of Cx43 and Cx45 in myocardial tissues of experimental animals were detected by Western blotting, while the corresponding myocardial tissues were stained with hematoxylin-eosin （HE） staining and Masson's staining, then histologically observed. Results Western blotting results showed that, compared with the control group, Cx43 expression in myocardial tissues of overworked rats decreased while Cx45 expression increased. HE staining and Masson's staining results showed that hypertrophy, rupture and interstitial fiber tissue hyperplasia were observed in myocardial fibers of overworked rats. Conclusion Overwork stress response may affect cardiac function as an independent factor and may even cause heart failure or arrhythmias and lead to death.
