Genetic Structure Analysis of Y-Chromosome STR and SNP in Population of Wujiang Area, Suzhou City.
10.12116/j.issn.1004-5619.2019.04.014
- Author:
Ze WU
1
;
Tian Fa CHEN
2
;
Zhi Fan ZENG
2
;
Yi Wei ZHANG
1
;
Zhen TANG
1
;
Kai Yuan SU
1
;
Chen Yun FAN
1
;
Shi Lin LI
1
Author Information
1. School of Life Sciences, Fudan University, Shanghai 200438, China.
2. Wujiang District Public Security Bureau of Suzhou, Suzhou 215200, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
short tandem repeat;
single nucleotide polymorphism;
Y chromosome;
genetic structures;
haplogroup;
Han nationality;
Suzhou
- MeSH:
China;
Chromosomes, Human, Y/genetics*;
Cities;
Gene Frequency;
Genetics, Population;
Haplotypes;
Humans;
Male;
Microsatellite Repeats;
Polymorphism, Single Nucleotide
- From:
Journal of Forensic Medicine
2019;35(4):448-454
- CountryChina
- Language:English
-
Abstract:
Objective To analyze the genetic phenotypes of Y-chromosome STR and SNP in Han male population of Wujiang area, Suzhou City and explore the genetic structure of population of Wujiang area for further examination of regional-specific Y-SNP genetic markers ancestor haplogroups. Methods Blood samples of 472 Wujiang area Han males were randomly collected and genotyped by YfilerTM Plus PCR Amplification Kit. The allele frequencies and haplotype frequencies of each locus were obtained using the direct calculation method. Y-SNP haplogroups of each sample were estimated using Y-Predictor software and verified through experiments by amplification refractory mutation system-polymerase chain reaction （ARMS-PCR）. Results A total of 453 haplotypes were found in the 27 Y-STR genetic markers in 472 Han males of Wujiang area. The haplotype diversity （HD） was 0.997 696 93, among which, the highest gene diversity （GD） value was DYF387S1a/b （GD=0.953 1） and the lowest was DYS438 （GD=0.321 8）. Based on genotyping data of 27 Y-STRs and 472 samples, 132 haplogroups from C, D, N, O and Q, etc downstream Y-SNP haplogroups were estimated and then verified through experiments. Conclusion This study is based on Y-chromosome STR haplotypes, and predicts Y-SNP haplogroups by Y-Predictor software, then uses ARMS-PCR to verify. Y-SNP genetic markers were introduced to achieve precise analysis of the genetic structure of male families in population of three towns in Wujiang area.
