Determination of the Content of 4-FMA in Rat Plasma Samples by HPLC-MS/MS Method.
10.12116/j.issn.1004-5619.2019.04.007
- Author:
Qian ZHENG
1
;
Mei Qiang WEN
1
;
Juan JIA
1
;
Tao WANG
1
;
Zhi Wen WEI
1
;
Hong Juan MA
1
;
You Mei WANG
2
;
Peng XU
2
;
Ke Ming YUN
1
Author Information
1. Shanxi Medical University, Taiyuan 030001, China.
2. National Narcotics Laboratory, Drug Intelligence and Forensic Center of Ministry of Public Security, Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
forensic toxicology;
high performance liquid chromatography-tandem mass spectrometry;
4-fluoromethamphetamine;
plasma;
rats
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Limit of Detection;
Methamphetamine/blood*;
Rats;
Reproducibility of Results;
Tandem Mass Spectrometry;
Toxicokinetics
- From:
Journal of Forensic Medicine
2019;35(4):419-422
- CountryChina
- Language:English
-
Abstract:
Objective To develop a high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） method for the determination of the content of 4-fluoromethamphetamine （4-FMA） in rat plasma, and to provide a methodological basis for the study of the toxicokinetics of 4-FMA in rats. Methods Rat plasma samples were added into internal standard methamphetamine （MA）. Its proteins were precipitated with methanol and then separated with Poroshell 120 EC-C18 chromatographic column. A 0.1% formic acid aqueous solution and a 0.1% formic acid acetonitrile solution were used as the mobile phase at the flow rate of 0.4 mL/min. Electrospray ionization source was used for detection in the multiple reaction monitoring （MRM） mode. Results The linear relationship was good when the mass concentration of 4-FMA in plasma samples was in the range of 5-1 000 ng/mL （r>0.999）. The limit of detection （LOD） was 3 ng/mL and the limit of quantification （LOQ） was 5 ng/mL. The accuracy was expressed as relative error （RE）, and in the range of ±5%, the intra-day precision and inter-day precision （relative standard deviation, RSD） less than 9%, and the extraction recovery rate was more than 90%. The analysis and detection of plasma samples were completed within 2.5 min. Conclusion This study developed a HPLC-MS/MS method for the determination of 4-FMA in rat plasma samples. This method is accurate, rapid, simple and sensitive and can be applied to the study of toxicokinetics of 4-FMA.
