Establishment and Its Application of UPLC-MS/MS Method for 20 Fentanyl-related Substances in Blood.
10.12116/j.issn.1004-5619.2019.04.006
- Author:
Yan SHI
1
;
Huo Sheng QIANG
1
;
Wei LIU
1
;
Ping XIANG
1
;
Bao Hua SHEN
1
;
Min SHEN
1
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
forensic toxicology;
fentanyl analogues;
ultra-high performance liquid chromatography-tandem mass spectrometry;
blood
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Fentanyl/blood*;
Forensic Toxicology;
Rats;
Reproducibility of Results;
Sufentanil/blood*;
Tandem Mass Spectrometry
- From:
Journal of Forensic Medicine
2019;35(4):411-418
- CountryChina
- Language:English
-
Abstract:
Objective To establish an ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） rapid determination method for simultaneous analysis of 20 fentanyl-related substances in blood. Methods With fentanyl-D5 as an internal standard, the blood was extracted by liquid-liquid extraction （LLE）, then separated with an ACQUITY UPLC HSS T3 chromatographic column, and finally 20 fentanyl-related substances were simultaneously analyzed with multiple reaction monitoring （MRM） mode. Results The limits of detection （LOD） of all compounds were 0.02-0.03 ng/mL, and the limits of quantitation （LOQ） were 0.05-0.2 ng/mL. Within the mass concentration range of 0.05-40 ng/mL, 20 fentanyl-related substances had a good linear relationship, and correlation coefficients were larger than 0.99. The accuracy of the method was 87.69%-114.68% and the extraction recovery rate was 85.35%-101.80%, and no significant matrix effect was observed. The established method was successfully applied to the detection of sufentanil in rat blood after sufentanil was injected. Sufentanil could still be detected in blood of rats 10 h after sufentanil injection. Conclusion The established method has the advantages of simple pretreatment, high sensitivity and good selectivity, and can be used for the determination of fentanyl-related substances in forensic toxicology analysis.
