Network Analysis of Y-STR in Six Ethnic Populations in Guangxi and Its Forensic Significances.
10.12116/j.issn.1004-5619.2019.03.010
- Author:
Yue XIAO
1
;
Pan DENG
2
;
Kai Chuang CHANG
3
;
Quan MA
4
;
En Fang QIAN
4
;
Jian Hua YU
5
;
Bao Wen CHENG
6
;
Cai Xia LI
4
;
Li JIANG
4
Author Information
1. Guangdong Zhongyi Forensic Science Center, Shenzhen 518000, Guangdong Province, China.
2. Zhuzhou Municipal Public Security Bureau, Zhuzhou 412007, Hunan Province, China.
3. Kunming Municipal Public Security Bureau, Kunming 650034, China.
4. Key Laboratory of Forensic Genetics, National Engineering Laboratory for Forensic Science, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Ministry of Public Security, PRC, Beijing 100038, China.
5. Zhaotong Municipal Public Security Bureau, Zhaotong 657000, Yunnan Province, China.
6. Yunnan Police Officer Academy, Kunming 650221, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
polymorphism, single nucleotide;
Y chromosome;
short tandem repeat;
haplotype;
Guangxi
- MeSH:
China;
Chromosomes, Human, Y;
Ethnicity;
Genetics, Population;
Haplotypes;
Humans;
Male;
Microsatellite Repeats
- From:
Journal of Forensic Medicine
2019;35(3):314-318
- CountryChina
- Language:English
-
Abstract:
Objective To explore the distribution of genetic structure of Y-SNP and Y-STR genetic markers in different ethnic groups and its application in forensic science. Methods SNaPshot minisequencing was used to detect the polymorphisms of 12 Y-SNP loci in 439 males from 6 ethnic groups, including Guangxi Han, Guangxi Jing, Guangxi Miao, Guangxi Yao, Guangxi Zhuang and Guangxi Dong. DNATyperTM Y26 kit was used to multiplex-amplify 26 Y-STR loci. The PCR products were analyzed by 3130xl genetic analyzer. The network analysis of Y-STR haplotype under the same Y-SNP haplogroup was analyzed by Network 5.0 software. Results Six haplogroups defined by 12 Y-SNP loci were detected in 6 ethnic groups, and 362 haplotypes were detected in 26 Y-STR loci. The haplotype diversity was 0.996 6. In the C haplogroup, the samples from Guangxi Yao, Guangxi Zhuang and Guangxi Dong were clustered on different branches; in the O1 haplogroup, those from Guangxi Zhuang, Guangxi Miao and Guangxi Jing were relatively independent and clustered separately; in the O2 haplogroup, some samples from Guangxi Miao and Guangxi Yao were gathered in a cluster. Conclusion Based on the Y-STR network analysis of samples with identical haplogroup of Y-SNP, some ethnic groups can be preliminarily distinguished, which could be used to infer male suspects' ethnic group through detecting their genetic markers left in the crime scene.
