Relationship between Changes of Pericyte Number and Wound Age during Repair of Skeletal Muscle Contusion in Mice.
10.12116/j.issn.1004-5619.2019.03.001
- Author:
Shu Heng WEN
1
;
Zhi Ling TIAN
2
;
Miao ZHANG
1
;
Meng Zhou ZHANG
1
;
Shuai WANG
1
;
Jing Wei CHEN
1
;
Ying Fu SUN
1
;
Chang Liang WANG
1
;
Rui ZHAO
1
;
Da Wei GUAN
1
Author Information
1. Center of Medico-legal Investigation, School of Forensic Medicine, China Medical University, Shenyang 110122, China.
2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
contusions;
satellite cells, skeletal muscle;
pericytes;
wound age estimation;
mice
- MeSH:
Animals;
Contusions;
Disease Models, Animal;
Mice;
Muscle, Skeletal;
Pericytes;
Rats, Sprague-Dawley
- From:
Journal of Forensic Medicine
2019;35(3):267-272
- CountryChina
- Language:English
-
Abstract:
Objective To observe the change pattern of pericyte number at different time periods after mice skeletal muscle contusion and discuss its role in wound age estimation. Methods A mice gastrocnemius muscle contusion model was established. The form and number changes of pericytes at 1, 3, 5, 7, 9, 14, and 28 d post-injury were detected by multiple immunofluorescence staining. Results Compared with the slender shape of pericytes in normal skeletal muscles, pericytes in the contusion area had increased volume, rounder form and a round nuclei. Part of pericytes were found to express satellite cell markers paired-box transcription factor （Pax7） or myoblast determination 1 （MyoD1）. The changes of pericyte number in skeletal muscles after contusion were time-dependant, and showed unimodal distribution with the extension of wound age. In the central contusion area, the number of pericytes peaked at 5 d post-injury while in the peripheral contusion area, the number of pericytes peaked at 5 d and 7 d post-injury. Conclusion The number of pericytes in contusion area varies time-dependently after skeletal muscle contusion in mice and might be a reference index for muscle wound age estimation, and is involved in the repair and regeneration of skeletal muscle injury.
