Wound Age Estimation by Neutrophil Migration Distance.
10.12116/j.issn.1004-5619.2019.02.007
- Author:
Qi Qing LIU
1
;
Hong Min GUO
2
;
Lei WANG
1
;
Han Lin LU
1
;
Qiu Xiang DU
1
;
Ru Feng BAI
3
;
Jun Hong SUN
1
;
Ying Yuan WANG
1
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
2. Yingze Branch of Taiyuan Public Security Bureau, Taiyuan 030002, China.
3. School of Criminal Justice, China University of Political Science, Beijing 100040, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
wounds and injuries;
cell migration assays;
neutrophil;
skeletal muscle;
wound age estimation;
rats
- MeSH:
Animals;
Contusions/metabolism*;
Follow-Up Studies;
Muscle, Skeletal/pathology*;
Neutrophil Infiltration;
Neutrophils;
Rats;
Rats, Sprague-Dawley;
Time Factors
- From:
Journal of Forensic Medicine
2019;35(2):166-170
- CountryChina
- Language:English
-
Abstract:
Objective To explore the application of neutrophil migration distance for wound age estimation of skeletal muscles in rats, and to provide methodological basis for follow-up study in future. Methods The skeletal muscle contusion model was established in rats, and the control group and the 2, 4, 6 h post-traumatic groups were set. The law of response of neutrophils that participated in the inflammation after injury was detected by immunohistochemical staining, and the relationship between neutrophil migration distance and injury time was detected by TissueFAXS PLUS software. Results The skeletal muscle was obviously infiltrated with neutrophils 2-6 h after injury. The positive rate of neutrophil was （28.75±0.94）% at 2 h post-traumatic, and reached the peak （45.50±3.63）% at 4 h post-traumatic, then decreased to （31.92±1.56）% at 6 h post-traumatic. The neutrophil migration distances increased with the progress of inflammation, and reached （124.80±12.32） μm, （229.03±21.45） μm and （335.04±16.75） μm at 2 h, 4 h and 6 h, respectively. Conclusion There is a relationship of neutrophil infiltrated number and migration distance and wound age within the 2-6 h after skeletal muscle injury, which could be used for the inference of skeletal muscle wound age.
