Expressions of TGF-β1 and EⅢA-FN after Rat Skeletal Muscle Contusion and Wound Age Estimation.
10.12116/j.issn.1004-5619.2019.02.005
- Author:
Ran LIU
1
;
Lu Zou GE
2
;
Hai Dong ZHANG
1
;
Dong ZHAO
1
;
Wei Liang HOU
1
;
Xiao Fei E
1
;
Tian Shui YU
1
Author Information
1. Collaborative Innovation Center of Judicial Civilization, Beijing 100088, China.
2. High District Branch of Weihai Public Security Bureau, Weihai 264200, Shandong Province, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
wounds and injuries;
skeletal muscle;
transforming growth factor β1;
fibronectin;
EⅢA segment;
wound age estimation;
rats
- MeSH:
Animals;
Biomarkers/metabolism*;
Contusions/metabolism*;
Fibroblasts;
Fibronectins/metabolism*;
Muscle, Skeletal/metabolism*;
Postmortem Changes;
Random Allocation;
Rats;
Transforming Growth Factor beta1/metabolism*
- From:
Journal of Forensic Medicine
2019;35(2):154-159
- CountryChina
- Language:English
-
Abstract:
Objective To study the expressions of transforming growth factor-β1 （TGF-β1） and EⅢA-fibronectin （EⅢA-FN） at different time points of antemortem injury, antemortem injury postmortem expression and postmortem injury and to explore their application value in wound age estimation. Methods A model of rat skeletal muscle contusion was established. The rats were randomly divided into normal control group （n=5）, antemortem contusion group （n=40）, antemortem contusion postmortem expression group （n=110） and postmortem injury group （n=25）. The expressions of TGF-β1 and EⅢA-FN after rat skeletal muscles antemortem contusion were detected with immunohistochemical staining. Expression changes of TGF-β1 and EⅢA-FN mRNA in each group were analyzed with real-time fluorescence quantitative PCR. Results Immunohistochemical staining results showed that a large number of polymorphonuclear leukocyte, mononuclear cells and fibroblastic cells showed a strong expression of TGF-β1 in wounded zones 12 h-14 d after antemortem contusion. EⅢA-FN was mainly distributed in the extracellular matrix, 3 to 7 d post-traumatic. Real-time fluorescence quantitative PCR results showed that TGF-β1 and EⅢA-FN mRNA in antemortem injury group reached the peak at 3 and 5 d post-traumatic respectively. The expressions of TGF-β1 and EⅢA-FN mRNA in antemortem contusion postmortem expression group peaked at 6 h and 12 h postmortem. The expression of TGF-β1 and EⅢA-FN mRNA in postmortem injury group 0.5-12 h postmortem was significantly lower than those of the normal control group and the antemortem contusion group. Conclusion TGF-β1 and EⅢA-FN might become a reference index for skeletal muscle wound age estimation.
