Application Prospect of Massively Parallel Sequencing in Mixed Stain Detection.
10.12116/j.issn.1004-5619.2019.01.016
- Author:
Jing LIU
1
;
Guang-lin HE
1
;
Yi-ping HOU
1
;
Zheng WANG
1
Author Information
1. nstitute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, China.
- Publication Type:Review
- Keywords:
forensic genetics;
mixed stain;
massively parallel sequencing;
second generation sequencing;
review
- MeSH:
Coloring Agents;
DNA Fingerprinting;
High-Throughput Nucleotide Sequencing;
Microsatellite Repeats;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2019;35(1):84-88
- CountryChina
- Language:English
-
Abstract:
Mixed stains is the common biological sample in sexual crime cases. Its analysis and DNA profiles interpretation are one of the difficulties in forensic examination. The current genetic marking of mixed stain detection mainly rely on capillary electrophoresis （CE） separation technology, and the analysis methods of the results are mainly inclusion rate and likelihood methods. Because CE has limited resolution and is not able to exploit the efficacy of each genetic marker, its ability to split mixed stain is limited. In recent years, the emerging massively parallel sequencing （MPS） technique not only can obtain the base sequence information of genetic markers, but also is capable of detecting multiple genetic markers simultaneously, and thus derives new analytical methods, bringing new opportunities for forensic detection and analysis of mixed stain. This paper intends to review the application prospects of conventional mixed stain analyses and MPS technique, therefore to provide references for later research and practice.
