Methylation-Based Age Estimation Model Construction and Its Effectiveness Evaluation.
10.12116/j.issn.1004-5619.2019.01.004
- Author:
Shan Fei LI
1
;
Fu Duan PENG
2
;
Jian Ning WANG
3
;
Jian Jun ZHONG
4
;
Hui ZHAO
5
;
Ling WANG
5
;
Yong Jiu LI
5
;
Fan LIU
2
;
Cai Xia LI
1
;
Lei FENG
5
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
2. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
3. Linyi Public Security Bureau, Linyi 044100, Shanxi Province, China.
4. Dezhou Public Security Bureau, Dezhou 253012, Shandong Province, China.
5. Key Laboratory of Forensic Genetics, Ministry of Public Security, National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Beijing 100038, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
forensic anthropology;
DNA methylation;
age determination;
linear model;
Han nationality;
northern China;
males
- MeSH:
Adaptor Proteins, Signal Transducing;
Apoptosis Regulatory Proteins;
Asian People;
Child, Preschool;
China;
CpG Islands;
DNA Methylation;
Humans;
Intracellular Signaling Peptides and Proteins;
Linear Models;
Male;
Membrane Proteins;
Metalloproteins;
Monomeric GTP-Binding Proteins;
Tripartite Motif Proteins
- From:
Journal of Forensic Medicine
2019;35(1):17-22
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To screen the DNA methylation loci associated with the age of Han males in northern China and to construct an age estimation model.
METHODS:Twenty-one candidate methylation loci were screened. The DNA methylation levels of 476 blood samples from Chinese Han males were detected for 21 amplicons using EpiTYPER technology platform, and data on 153 DNA methylation loci were obtained.
RESULTS:After correlation analysis, 8 age-related DNA methylation loci were finally screened. CpG1, CpG2, CpG4, CpG7, CpG8 were located on TRIM59, RASSF5, Clorf132, CSNK1D, ELOVL2,CpG5, CpG6 on PDE4C, and CpG3 on chr17:21452808. Based on the 8 loci, 352 samples were used for model construction. A multivariate linear regression age estimation model was constructed （R2=0.93）, with mean absolute deviation （MAD） of 2.69 years old. When 109 samples were used for model validation, the MAD was 3.80 years old. The test was repeated 3 times in 15 new samples, with MADs of 4.08, 4.68 and 3.93 years old, respectively.
CONCLUSIONS:The age estimation model on Han males in northern China constructed in this study can be used to estimate the age of victims and suspects and to narrow the scope of investigation, and therefore has practical application value.
