Rapid Determination of Cocaine and Its Metabolite Benzoylecgonine in Hair by LC-MS/MS.
10.12116/j.issn.1004-5619.2018.04.006
- Author:
Mei Ru PAN
1
;
Huo Sheng QIANG
2
;
Bao Hua SHEN
3
;
Hui YAN
3
;
Ping XIANG
3
Author Information
1. Shenyang Pharmaceutical University, Shenyang 110016, China.
2. Guangzhou Wondfo Biotech Co. Ltd, Guangzhou 510663, China.
3. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
benzoylecgonine;
cocaine;
forensic toxicology;
hair
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid/methods*;
Cocaine/metabolism*;
Hair/metabolism*;
Humans;
Reference Standards;
Reproducibility of Results;
Tandem Mass Spectrometry/methods*
- From:
Journal of Forensic Medicine
2018;34(4):375-378
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To establish a rapid determination method with LC-MS/MS for cocaine and its metabolite benzoylecgonine in hair.
METHODS:Deuterated internal standards （cocaine-D₃ and benzoylecgonine-D₈） were added to the decontaminated hair. After the extraction by ultrasonication with methanol, the compounds were separated by the Restek Allure PFP propyl column, and cocaine and benzoylecgonine were simultaneously analysed in multiple reaction monitoring mode.
RESULTS:The cocaine and benzoylecgonine in hair showed a good linearity in the range of mass fraction between 0.02 and 10.00 ng/mg with the limits of detection of 0.01 ng/mg.
CONCLUSIONS:The developed method is simple and rapid with a good selectivity, which is suitable for the determination of cocaine and its metabolite benzoylecgonine in hair.
