Determination of Sulfide Ion in Blood from Hydrogen Sulfide Poisoning Cases.
10.3969/j.issn.1004-5619.2017.02.008
- Author:
Huo Sheng QIANG
1
;
Hang CHEN
2
;
Bao Hua SHEN
2
;
Min SHEN
2
;
Ping XIANG
1
Author Information
1. Pharmacy School of Wenzhou Medical University, Wenzhou 325035, China.
2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, PRC, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
derivatization;
forensic toxicology;
gas chromatography-mass spectrometry;
hydrogen sulfide;
poisoning
- MeSH:
Fluorobenzenes;
Gas Chromatography-Mass Spectrometry/methods*;
Humans;
Hydrogen Sulfide/blood*;
Limit of Detection;
Sulfides
- From:
Journal of Forensic Medicine
2017;33(2):148-153
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To establish a gas chromatography-mass spectrometry （GC-MS） method for the determination of sulfide ion in blood and apply it to the practical cases.
METHODS:The 1, 3, 5-tribromobenzene was selected as an internal standard, and 0.2 mL blood sample was collected and analyzed using GC-MS after α-Bromo-2, 3, 4, 5, 6-pentafluorobenzyl bromide derivatization.
RESULTS:The mass concentration of sulfide ion in blood had good linearity in the range of 0.2-40 μg/mL with a limit of detection （LOD） of 0.05 μg/mL. The mass concentration of sulfide ion was less than 0.05 μg/mL in blank blood from different sources such as healthy subjects and dead cases. In 3 sulfide poisoning cases, sulfide ion was detected in the blood samples of 6 victims, and the mass concentration range was 1.02-3.13 μg/mL.
CONCLUSIONS:This study establishes a method for investigation of sulfide ion in blood which has been applied successfully to the cases of fatal sulfide poisonings.
