Full Sibling Identification by IBS Scoring Method and Establishment of the Query Table of Its Critical Value.
10.3969/j.issn.1004-5619.2017.02.006
- Author:
Ran LI
1
;
Cheng Tao LI
2
;
Shu Ming ZHAO
3
;
Hai Xia LI
1
;
Li LI
1
;
Ri Ga WU
1
;
Chu Chu ZHANG
1
;
Hong Yu SUN
1
Author Information
1. Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China.
2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, PRC, Shanghai 200063, China.
3. Shanghai Chromysky Medical Research Co., Ltd, Shanghai 201200, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
identity by state （IBS）;
short tandem repeat;
sibling relations
- MeSH:
Alleles;
Genotype;
Humans;
Irritable Bowel Syndrome/genetics*;
Reproducibility of Results;
Research Design;
Siblings
- From:
Journal of Forensic Medicine
2017;33(2):136-140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To establish a query table of IBS critical value and identification power for the detection systems with different numbers of STR loci under different false judgment standards.
METHODS:Samples of 267 pairs of full siblings and 360 pairs of unrelated individuals were collected and 19 autosomal STR loci were genotyped by Goldeneye™ 20A system. The full siblings were determined using IBS scoring method according to the 'Regulation for biological full sibling testing'. The critical values and identification power for the detection systems with different numbers of STR loci under different false judgment standards were calculated by theoretical methods.
RESULTS:According to the formal IBS scoring criteria, the identification power of full siblings and unrelated individuals was 0.764 0 and the rate of false judgment was 0. The results of theoretical calculation were consistent with that of sample observation. The query table of IBS critical value for identification of full sibling detection systems with different numbers of STR loci was successfully established.
CONCLUSIONS:The IBS scoring method defined by the regulation has high detection efficiency and low false judgment rate, which provides a relatively conservative result. The query table of IBS critical value for identification of full sibling detection systems with different numbers of STR loci provides an important reference data for the result judgment of full sibling testing and owns a considerable practical value.
