Forensic Application of HuaxiaTM Platinum Kit.
10.3969/j.issn.1004-5619.2017.02.005
- Author:
Ya Li WANG
1
;
Xiang SHENG
2
;
Min LI
2
;
Yu Ling CHEN
2
;
Yuan LIN
2
;
Li Qin CHEN
1
Author Information
1. Department of Forensic Medicine, Inner Mongolia Medical University, Hohhot 010030, China.
2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, PRC, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
Han nationality;
Huaxia™ Platinum kit;
forensic genetics;
polymorphism, genetic;
short tandem repeat
- MeSH:
Alleles;
Asian People/genetics*;
China;
Forensic Genetics/methods*;
Gene Frequency;
Genetics, Population;
Genotype;
Humans;
Microsatellite Repeats;
Paternity;
Platinum;
Polymorphism, Genetic;
Probability;
Reagent Kits, Diagnostic
- From:
Journal of Forensic Medicine
2017;33(2):129-135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the genetic polymorphism of 23 autosomal STR loci of Huaxia™ Platinum kit in Chinese Han population, and to evaluate the forensic efficiency of Huaxia™ Platinum kit.
METHODS:A total of 500 unrelated healthy individuals from Han population were genotyped with Huaxia™ Platinum kit. The frequency distribution and the parameter of population genetics of STR loci were analysed statistically. Huaxia™ Platinum kit was compared with other 7 commercial STR kits commonly seen at home and abroad in the number of STR loci, interior label, fluorescent mark, total number of alleles in Ladder and system effectiveness.
RESULTS:All the 23 autosomal STR loci were consistent with Hardy-Weinberg equilibrium （P>0.05）. The discrimination power was 0.791 5-0.986 2. The polymorphism information content （PIC） was 0.559 0-0.914 0. The combined discrimination power （CDP） was 1-4.1×10⁻²⁸, while combined probability of paternity exclusion in trio （CPET） and in duo （CPED） were 1-4.1×10⁻¹⁰ and 1-8.4×10⁻⁷, respectively. Compared with other 7 kits, Huaxia™ Platinum kit contained the most number of alleles within the Ladder.
CONCLUSIONS:All the 23 autosomal STR loci of Huaxia™ Platinum kit with highly polymorphic in Han population can be used for paternity testing and individual identification. Compared with other 7 kits, it appears that Huaxia™ Platinum kit can provide more genetic information.
