Establishment of Automation System for Detection of Alcohol in Blood.
10.3969/j.issn.1004-5619.2017.01.006
- Author:
Lin Lin TIAN
1
;
Lei SHEN
1
;
Jin Feng XUE
1
;
Ming Ming LIU
1
;
Li Jun LIANG
1
Author Information
1. Criminal Police Branch, Jiaxing Public Security Bureau, Jiaxing 314000, China.
- Publication Type:Journal Article
- Keywords:
automation system;
blood;
ethanol;
forensic toxicology;
headspace gas chromatography
- MeSH:
Automation;
Chromatography, Gas/methods*;
Ethanol/blood*;
Gas Chromatography-Mass Spectrometry/methods*
- From:
Journal of Forensic Medicine
2017;33(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To establish an automation system for detection of alcohol content in blood.
METHODS:The determination was performed by automated workstation of extraction-headspace gas chromatography （HS-GC）. The blood collection with negative pressure, sealing time of headspace bottle and sample needle were checked and optimized in the abstraction of automation system. The automatic sampling was compared with the manual sampling.
RESULTS:The quantitative data obtained by the automated workstation of extraction-HS-GC for alcohol was stable. The relative differences of two parallel samples were less than 5%. The automated extraction was superior to the manual extraction. A good linear relationship was obtained at the alcohol concentration range of 0.1-3.0 mg/mL （r≥0.999） with good repeatability.
CONCLUSIONS:The method is simple and quick, with more standard experiment process and accurate experimental data. It eliminates the error from the experimenter and has good repeatability, which can be applied to the qualitative and quantitative detections of alcohol in blood.
