Metabolic Characteristics of Lethal Bradycardia Induced by Myocardial Ischemia.
10.3969/j.issn.1004-5619.2017.01.003
- Author:
Jia Yan WU
1
;
Dian WANG
1
;
Jing KONG
1
;
Xing Xing WANG
1
;
Xiao Jun YU
1
Author Information
1. Department of Forensic Pathology, Medical College, Shantou University, Shantou 515041, China.
- Publication Type:Journal Article
- Keywords:
bradycardia;
forensic pathology;
metabolic;
myocardial ischemia;
serum
- MeSH:
Animals;
Bradycardia/pathology*;
Coronary Artery Disease;
Death, Sudden, Cardiac;
Disease Models, Animal;
Gas Chromatography-Mass Spectrometry/methods*;
Lysine/metabolism*;
Metabolomics/methods*;
Myocardial Ischemia/metabolism*;
Purines/metabolism*;
Rats
- From:
Journal of Forensic Medicine
2017;33(1):11-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To explore the metabolic characteristics of lethal bradycardia induced by myocardial ischemia in rat's serum.
METHODS:A rat myocardial ischemia-bradycardia-sudden cardiac death （MI-B-SCD） model was established, which was compared with the sham-operation group. The metabolic profile of postmortem serum was analyzed by gas chromatography-mass spectrometry （GC-MS）, coupled with the analysis of serum metabolic characteristics using metabolomics strategies.
RESULTS:The serum metabolic profiles were significantly different between the MI-B-SCD rats and the control rats. Compared to the control rats, the MI-B-SCD rats had significantly higher levels of lysine, ornithine, purine, serine, alanine, urea and lactic acid; and significantly lower levels of succinate, hexadecanoic acid, 2-ketoadipic acid, glyceraldehyde, hexendioic acid and octanedioic acid in the serum. There were some correlations among different metabolites.
CONCLUSIONS:There is obvious metabolic alterations in the serum of MI-B-SCD rat. Both lysine and purine have a high value in diagnosing MI-B-SCD. The results are expected to provide references for forensic and clinical applications of prevention and control of sudden cardiac death.
