Individual Identification of Cartilage by Direct Amplification in Mass Disasters.
10.3969/j.issn.1004-5619.2017.03.014
- Author:
Chuan Hai WANG
1
;
Cheng XU
1
;
Xiang Qin LI
1
;
Yong WU
1
;
Zhou DU
1
Author Information
1. Criminal Technology Division, Criminal Police Detachment, Shenzhen Public Security Bureau, Shenzhen 518040, China.
- Publication Type:Journal Article
- Keywords:
cartilage;
direct amplification;
forensic genetics;
genotyping techniques;
individual identification
- MeSH:
Cartilage;
DNA/genetics*;
DNA Fingerprinting/methods*;
Disasters;
Genotype;
Humans;
Microsatellite Repeats/genetics*;
Molecular Weight;
Polymerase Chain Reaction
- From:
Journal of Forensic Medicine
2017;33(3):281-283
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To explore the effectiveness of direct amplification for the STR analysis of cartilage, and to accelerate the effectiveness of disaster victim identification.
METHODS:Eighty-eight cartilage samples were directly amplified by PowerPle® 21 kit, and the results of genotyping were compared with that obtained by the magnetic beads method.
RESULTS:In 88 cartilage samples, the STR genotypes were successfully detected from 84 samples by direct amplification and magnetic beads method, and both the results of genotyping by two method were consistent.
CONCLUSIONS:Direct amplification with PowerPlex® 21 kit can be used for STR genotyping of cartilages. This method is operated easily and promptly, which has a potential application in the individual identification of mass disasters.
