Genotyping of ABO Blood Group in Partial Population of Yunnan Province by SNaPshot Technology.
10.3969/j.issn.1004-5619.2017.03.013
- Author:
Shu Xin YU
1
;
Fa Ming ZENG
1
;
Yan Zhang JIN
2
;
Hong Jing WAN
3
;
Dian ZHAI
4
;
Yu Ming XING
1
;
Bao Wen CHENG
1
Author Information
1. Kunming Medical University, Kunming 650500, China.
2. Longchuan Public Security Bureau, Longchuan 678700, China.
3. Lijiang Public Security Bureau,Lijiang 674100, China.
4. Yunnan Provincial Public Security Department, Kunming 650228, China.
- Publication Type:Journal Article
- Keywords:
ABO blood group system;
SNaPshot technology;
forensic genetics;
genetic testing
- MeSH:
ABO Blood-Group System/genetics*;
Alleles;
Asian People/genetics*;
China;
DNA;
Ethnicity;
Gene Frequency;
Genotype;
Humans;
Polymerase Chain Reaction/methods*;
Polymorphism, Restriction Fragment Length
- From:
Journal of Forensic Medicine
2017;33(3):277-280
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To detect the genotype of ABO blood group by SNaPshot technology.
METHODS:DNA were extracted from the peripheral blood samples with known blood groups （obtained by serology） of 107 unrelated individuals in Yunnan. Six SNP loci of the 261th, 297th, 681th, 703th, 802th, and 803th nucleotide positions were detected by SNaPshot Multiplex kit, and relevant genetics parameters were calculated.
RESULTS:In 107 blood samples, the allele frequencies of types A, B, OA, and OG were 0.355 1, 0.168 2, 0.230 0 and 0.247 6, respectively, while that of types AG and cis AB were not detected. The genotyping results of ABO blood group were consistent with that of serologic testing.
CONCLUSIONS:SNaPshot technology can be adapted for genotyping of ABO blood group.
