Genetic Polymorphisms of 30 InDel Loci in Ewenki Ethnic Group from Inner Mongolia.
10.3969/j.issn.1004-5619.2017.03.012
- Author:
Xiao Ye JIN
1
;
Yuan Yuan WEI
1
;
Yong Feng HE
2
;
Yu Xin GUO
1
;
Ting MEI
3
;
Hao Tian MENG
1
;
Yu Dang ZHANG
4
;
Ting Ting KONG
1
;
Bo Feng ZHU
1
Author Information
1. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.
2. Department of Criminal Investigation, Shaanxi Provincial Public Security Bureau, Xi'an 710016, China.
3. Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830011, China.
4. Institute of Forensic Science of Anhui Public Security Department, Hefei 230061, China.
- Publication Type:Journal Article
- Keywords:
Ewenki ethnic group;
Inner Mongolia;
forensic genetics;
insertion/deletion;
polymorphism,genetic
- MeSH:
Asian People/genetics*;
Beijing;
China/epidemiology*;
DNA;
Ethnicity/genetics*;
Gene Frequency;
Genetic Loci;
Genetics, Population;
Genotype;
Humans;
INDEL Mutation;
Linkage Disequilibrium;
Microsatellite Repeats;
Phylogeny;
Polymorphism, Genetic;
Social Behavior
- From:
Journal of Forensic Medicine
2017;33(3):271-276
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To study the genetic polymorphisms of 30 insertion/deletion （InDel） loci and evaluate their forensic application in Ewenki ethnic group from Inner Mongolia.
METHODS:Peripheral blood samples were collected from 87 unrelated healthy individuals in Ewenki ethnic group. Genomic DNA were extracted, and 30 InDel loci of the samples were multiplex amplified and genotyped. Hardy-Weinberg balance tests were preformed for all loci and genetic parameters were calculated by modified PowerStats v1.2 software. The linkage disequilibrium between loci were tested by SNPAnalyzer v2.0 software. Based on the allele frequencies of 30 InDel loci, the genetic relationships between Ewenki ethnic group and other populations were evaluated by analysis of molecular variance, principal component analysis and phylogenetic reconstruction.
RESULTS:After correction, 30 InDel loci conformed to Hardy-Weinberg equilibrium. It was found that the pairwise InDel loci were in linkage equilibrium after Bonferroni correction. The results of population genetics indicated that Ewenki ethnic group had close genetic relationships with Henan Han and Beijing Han populations; whereas it was significantly different from several populations in Europe and Mexico.
CONCLUSIONS:There are relatively high genetic polymorphisms on 30 InDel loci of Ewenki ethnic group from Inner Mongolia, which can be used as a helpful supplement application for STR detection system.
