The Changes of HIF-1α and VEGF-A in Myocardial Tissue of Rats with Arrhythmias.
10.3969/j.issn.1004-5619.2017.03.002
- Author:
Yuan ZHANG
1
;
Zhi Peng CAO
1
;
Rui Ming MAO
1
;
Zhong Bo DU
1
;
Li MI
1
;
Xin Yi LUO
1
;
Mei Hui TIAN
1
;
Bao Li ZHU
1
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110122, China.
- Publication Type:Journal Article
- Keywords:
arrhythmias, cardiac;
forensic pathology;
hypoxia inducible factor 1;
myocardium;
rats;
vascular endothelial growth factor-A
- MeSH:
Animals;
Arrhythmias, Cardiac/metabolism*;
Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*;
Myocardial Ischemia/metabolism*;
Myocardium/metabolism*;
Rats;
Vascular Endothelial Growth Factor A/metabolism*
- From:
Journal of Forensic Medicine
2017;33(3):225-231
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To observe the expression changes of hypoxia inducible factor-1α（HIF-1α） and vascular endothelial growth factor-A （VEGF-A） in rats with arrhythmias, and to explore the differences of the expression pattern in the two indicators of acute myocardial ischemia caused by arrhythmias and coronary insufficiency.
METHODS:The arrhythmia was induced by CaCl₂, and the expression changes of HIF-1α and VEGF-A were detected by immunohistochemistry, Western blotting and real-time PCR within 6 h after the arrhythmia in rats.
RESULTS:The expression of HIF-1α and VEGF-A showed diffuse in the myocardial tissue of rats died from arrhythmias. Both of them increased in the early arrhythmia, then decreased. Extensive myocardial ischemia happened at the beginning of arrhythmia occurrence and its range didn't expand with time.
CONCLUSIONS:The expressions of HIF-1α and VEGF-A in myocardium of the rats with arrhythmia can provide evidence for the differential diagnosis of acute myocardial ischemia caused by fatal arrhythmia and coronary insufficiency.
