The Role of CBS in Injury Time Estimation after Brain Contusion.
10.3969/j.issn.1004-5619.2017.03.001
- Author:
Yang CHU
1
;
Guo Xian HAN
2
;
Yao Qi WANG
3
;
Hai Yan SHAN
4
;
Xi Ping CHEN
1
;
Lu Yang TAO
1
;
Ming Yang ZHANG
1
Author Information
1. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.
2. Ying- kou Public Security Bureau, Yingkou 115000, China.
3. Forensic Identification Center of Yellow River Hospital, Zhengzhou 450004, China.
4. Department of Obstetrics and Gynecology, North District of Suzhou Municipal Hospital, Suzhou 215008, China.
- Publication Type:Journal Article
- Keywords:
brain injuries;
cystathionine beta-synthase;
forensic pathology;
injury time;
mice
- MeSH:
Animals;
Blotting, Western;
Brain;
Brain Contusion/pathology*;
Brain Injuries/pathology*;
Cerebral Cortex/pathology*;
Cystathionine beta-Synthase/metabolism*;
Down-Regulation;
Immunohistochemistry;
Male;
Mice;
Time Factors
- From:
Journal of Forensic Medicine
2017;33(3):221-224
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To observe the changes of cystathionine β-synthase （CBS） expression in the cerebral cortex after brain contusion at different times.
METHODS:An experimental model of traumatic brain injury （TBI） in mice was established by an improved weight-drop device. Then Western blotting and immunohistochemical examination were used to detect the CBS expression in cerebral cortex around injury at different time points （1 h, 6 h, 12 h, 1 d, 2 d, 3 d, 7 d）.
RESULTS:The results of Western blotting revealed that the expression level of CBS was down-regulated and reached its lowest level at the 3rd days after injury, and then restored to normal level after 7 days. The results of immunohistochemistry showed that CBS was present in the normal brain cortex. CBS expression gradually decreased at the 3rd days after injury, and then restored to normal level after 7 days.
CONCLUSIONS:CBS has the potential to be a reference index for time estimation after brain contusion in forensic practice.
