Expression of XPG Gene in Forensic Age Estimation.
10.3969/j.issn.1004-5619.2016.06.005
- Author:
Xiao Dong DENG
1
;
Wei ZHANG
1
;
Bo ZHANG
1
;
Ying MA
2
;
Che Er MUER
1
;
Li Xia ZHANG
1
;
Ying XIE
1
;
Yun LIU
1
Author Information
1. Department of Forensic Medicine, North Sichuan Medical College, Nanchong 637000, China.
2. Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
- Publication Type:Journal Article
- Keywords:
age estimation;
enzyme linked immunosorbent assay;
forensic anthropology;
forensic genetics;
quantitative real-time PCR;
xeroderma pigmentosum group G
- MeSH:
Adult;
Age Factors;
Asian People;
DNA-Binding Proteins/genetics*;
Endonucleases/genetics*;
Forensic Genetics;
Humans;
Leukocytes, Mononuclear;
Middle Aged;
Nuclear Proteins/genetics*;
RNA, Messenger;
Real-Time Polymerase Chain Reaction;
Transcription Factors/genetics*;
Young Adult
- From:
Journal of Forensic Medicine
2016;32(6):415-419
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To explore the expression of xeroderma pigmentosum complementation group G （XPG） gene in healthy Han population of different ages and to analysis the relationship between the mRNA and protein expression levels of XPG and age, which may provide a new molecular-biological indicator for forensic age determination.
METHODS:Total 150 samples of peripheral blood were collected from healthy Han population of different ages. Total RNA of peripheral blood mononuclear cell （PBMC） were extracted by TRIzol method, and the relative expression of XPG mRNA in PBMC was detected by quantitative real-time PCR, and the protein expression levels of XPG in plasma were detected by ELISA.
RESULTS:The mRNA and protein expression levels of XPG in ≤18 years old group were significantly different from 19-45 years old group and ≥46 years old group （P<0.05）, while there was no significant difference between 19-45 years old group and ≥46 years old group （P>0.05）. No significant sex differences were observed in mRNA and protein expression levels of XPG （P>0.05）.
CONCLUSIONS:The relative expression level of XPG mRNA in PBMC declines with the increase of age in younger age, while the protein expression level in plasma increases with age, and XPG gene can be used as one of new markers for forensic age estimation.
