Relationship between the Expression of α-syn and Neuronal Apoptosis in Brain Cortex of Acute Alcoholism Rats.
10.3969/j.issn.1004-5619.2016.06.002
- Author:
Fan LI
1
;
Yue ZHANG
1
;
Shu Ling MA
1
Author Information
1. School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, China.
- Publication Type:Journal Article
- Keywords:
alcoholic intoxication;
alpha-synuclein;
apoptosis;
caspase-3;
cerebral cortex;
forensic pathology;
forensic toxicology;
rats
- MeSH:
Alcoholism/pathology*;
Animals;
Apoptosis;
Brain Edema/pathology*;
Caspase 3/metabolism*;
Cerebral Cortex/pathology*;
Ethanol;
Hypoxia/pathology*;
Neurons/pathology*;
Rats;
alpha-Synuclein/metabolism*
- From:
Journal of Forensic Medicine
2016;32(6):406-409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To observe the changes of expression of α-synuclein （α-syn） and neuronal apoptosis in brain cortex of acute alcoholism rats and to explore the mechanism of the damage caused by ethanol to the neurons.
METHODS:The model of acute alcoholism rat was established by 50% alcohol gavage. The α-syn and caspase-3 were detected by immunohistochemical staining and imaging analysis at 1 h, 3 h, 6 h and 12 h after acute alcoholism. The number of positive cell and mean of optical density were detected and the trend change was analyzed. The variance analysis and t-test were also performed.
RESULTS:The number of α-syn positive cell and average optical density in brain cortex of acute alcoholism rat increased significantly and peaked at 6 hour with a following slight decrease at 12 h, but still higher than the groups at 1 h and 3 h. Within 12 hours after poisoning, the number of caspase-3 positive cell and average optical density in brain cortex of rats gradually increased.
CONCLUSIONS:The abnormal aggregation of α-syn caused by brain edema and hypoxia may participate the early stage of neuronal apoptosis in brain cortex after acute alcoholism.
