Application of Multiple Displacement Amplification in Samples with Inhibitors.
10.3969/j.issn.1004-5619.2016.05.006
- Author:
Dong Xue DING
1
;
Mei DING
1
Author Information
1. Department of Forensic Serology, Faculty of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
hemoglobin;
humic acid;
inhibitors;
multiple displacement amplification;
nucleic acid amplification techniques
- MeSH:
DNA/analysis*;
Electrophoresis, Polyacrylamide Gel;
Forensic Medicine/methods*;
Hemoglobins;
Humans;
Humic Substances;
Nucleic Acid Amplification Techniques
- From:
Journal of Forensic Medicine
2016;32(5):342-345
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To explore the ability of inhibition resistibility of multiple displacement amplification （MDA） in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods （MBM） to purify sample.
METHODS:Different concentrations of hemoglobin and humid acid （HA） mixed with DNA samples and then divided the samples into MDA group, MBM group and control group. D3S1358 locus was amplified and detected by polyacrylamide gel electrophoresis detection system and AmpFℓSTR® Identifiler™ Plus Kit-capillary electrophoresis detection system.
RESULTS:When hemoglobin concentrations exceed 1 ng/μL or HA concentrations exceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.
CONCLUSIONS:MDA has the capability to remove the inhibition of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.
