Determination of Bloodstain Age by UV Visible Integrating Sphere Reflection Spectrum.
10.3969/j.issn.1004-5619.2016.05.002
- Author:
Li Qiang YAN
1
;
Ye GAO
1
Author Information
1. Department of Technology, Liaoning Police Academy, Dalian 116036, China.
- Publication Type:Journal Article
- Keywords:
blood stain;
forensic pathology;
integrating sphere;
spectrum analysis
- MeSH:
Blood Stains;
Forensic Sciences;
Humans;
Reference Standards;
Reproducibility of Results;
Spectrum Analysis/methods*;
Time Factors;
Ultraviolet Rays
- From:
Journal of Forensic Medicine
2016;32(5):326-328
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To establish a method for rapid identification of bloodstain age.
METHODS:Under laboratory conditions （20 ℃, 25 ℃ and 30 ℃）, an integrating sphere ISR-240A was used as a reflection accessory on an UV-2450 UV-vis spectrophotometer, and a standard white board of BaSO₄ was used as reference, the reflection spectrums of bloodstain from human ears' venous blood were measured at regular intervals. The reflection radios R₅₄₁ and R₅₇₇ at a specific wavelength were collected and the value of R₅₄₁/R₅₇₇ was calculated. The linear fitting and regression analysis were done by SPSS 17.0.
RESULTS:The results of regression analysis showed that R² of the ratios of bloodstain age to UV visible reflectivity in specific wavelengths were larger than 0.8 within 8 hours and under certain circumstances. The regression equation was established. The bloodstain age had significant correlation with the value of R₅₄₁/R₅₇₇.
CONCLUSIONS:The method of inspection is simple, rapid and nondestructive with a good reliability, and can be used to identify the bloodstain age within 8 hours elapsed-time standards under laboratory conditions.
