Expression of AQP-1 and AQP-4 in the Lungs of Drown Rats.
10.3969/j.issn.1004-5619.2016.05.001
- Author:
Bin ZHAO
1
;
Shi Qiang YAO
1
;
Xiao Hui HAO
2
Author Information
1. Forensic Science Brigade of Tangshan Public Security Bureau, Tangshan 063000, China.
2. Medical Research Center, North China University of Science and Technology, Tangshan 063000, China.
- Publication Type:Journal Article
- Keywords:
aquaporin-1;
aquaporin-4;
drowning;
forensic pathology;
rats
- MeSH:
Animals;
Aquaporin 1/metabolism*;
Aquaporin 4/metabolism*;
Autopsy;
Blotting, Western;
Drowning;
Immunohistochemistry;
Lung/metabolism*;
Male;
RNA, Messenger;
Rats;
Rats, Sprague-Dawley;
Rats, Wistar;
Real-Time Polymerase Chain Reaction
- From:
Journal of Forensic Medicine
2016;32(5):321-325
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To observe the changes of expression of aquaporin-1（AQP-1） and AQP-4 in drowned and postmortem immersed rats' lungs.
METHODS:Thirty healthy male Wistar rats were randomly divided into drowning group, postmortem immersion group and cervical dislocation group. The morphological changes of rats' lungs were observed using HE staining. The mRNA and protein expressions of AQP-1 and AQP-4 in rats' lungs were detected by real-time PCR, immunohistochemistry and Western blotting, respectively.
RESULTS:The results of immunohistochemistry and the Western blotting showed that the protein expression of AQP-1 of the drowning group was higher than the postmortem immersion group and the cervical dislocation group （P<0.05）. The result of immunohistochemistry showed that the protein expression of AQP-4 of the drowning group was higher than the postmortem immersion group and the cervical dislocation group （P<0.05） while no difference were detected among the three of them by Western blotting （P>0.05）. The mRNA expressions of AQP-1 and AQP-4 in rats' lungs of the drowning group was significantly higher than the postmortem immersion group （P<0.05）.
CONCLUSIONS:The increase of mRNA and protein expressions of AQP-1 and AQP-4 in lungs of rats with cute lung injury of the drowning group would be useful for differentiating vital drowning from postmortem immersion.
