Review of Second Generation Sequencing and Its Application in Forensic Genetics.
10.3969/j.issn.1004-5619.2016.04.012
- Author:
Su Hua ZHANG
1
;
Ying Nan BIAN
1
;
Qi ZHAO
1
;
Cheng Tao LI
1
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China.
- Publication Type:Review
- Keywords:
forensic genetics;
genetic markers;
review [publication type];
second generation sequencing
- MeSH:
DNA, Mitochondrial/genetics*;
Forensic Genetics;
Genetic Markers;
Humans;
MicroRNAs/genetics*;
Polymerase Chain Reaction;
Polymorphism, Single Nucleotide;
RNA, Messenger/genetics*;
Sequence Analysis, DNA/methods*
- From:
Journal of Forensic Medicine
2016;32(4):282-289
- CountryChina
- Language:Chinese
-
Abstract:
The rapid development of second generation sequencing （SGS） within the past few years has led to the increasement of data throughput and read length while at the same time brought down substantially the sequencing cost. This made new breakthrough in the area of biology and ushered the forensic genetics into a new era. Based on the history of sequencing application in forensic genetics, this paper reviews the importance of sequencing technologies for genetic marker detection. The application status and potential of SGS in forensic genetics are discussed based on the already explored SGS platforms of Roche, Illumina and Life Technologies. With these platforms, DNA markers （SNP, STR）, RNA markers （mRNA, microRNA） and whole mtDNA can be sequenced. However, development and validation of application kits, maturation of analysis software, connection to the existing databases and the possible ethical issues occurred with big data will be the key factors that determine whether this technology can substitute or supplement PCR-CE, the mature technology, and be widely used for cases detection.
