Simultaneous Determination of Three Kinds of Effective Constituents in Cannabis Plants by Reversed-phase HPLC.
10.3969/j.issn.1004-5619.2016.04.006
- Author:
Qiang FU
1
;
Zhi SHU
2
;
Ke DENG
1
;
Xuan LUO
1
;
Chang Guang ZENG
1
Author Information
1. Criminal Police Branch, Deyang Public Security Bureau, Deyang 618000, China.
2. Lincang Public Security Bureau, Lincang 677000, China.
- Publication Type:Journal Article
- Keywords:
Cannabis;
cannabidiol;
cannabinol;
forensic toxicology;
high performance liquid chromatography;
tetrahydrocannabinol
- MeSH:
Cannabidiol/analysis*;
Cannabinol/analysis*;
Cannabis/chemistry*;
Chromatography, High Pressure Liquid;
Dronabinol/analysis*
- From:
Journal of Forensic Medicine
2016;32(4):261-263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To establish a high performance liquid chromatographic （HPLC） method for simultaneous determination of three effective constituents, including tetrahydrocannabinol （THC）, cannabidiol （CBD） and cannabinol （CBN） in Cannabis plants.
METHODS:A C₁₈ column was used in this study, and acetonitrile-phosphate buffer （0.015 mol/L KH₂PO₄） was used as mobile phase at a flow rate of 1.0 mL/min. At a detection wavelength of 220 mm, UV absorption spectra were collected at the wavelength range of 190-400 nm, and the spectra and retention time were counted as qualitative evidence.
RESULTS:THC, CBD and CBN could be well separated by this method. Three components had good linear relationship in the range of 0.4-40 μg/mL （R²≥0.999 3）. The recoveries were over 87%. The limits of detection were 1.8 ng, 2.0 ng and 1.3 ng, respectively. The relative standard deviation （RSD） were less than 5% for both inter-day and intra-day precisions.
CONCLUSIONS:Reversed-phase HPLC method is simple, rapid and accurate, and it is suitable for the qualitative and quantitative detection of THC, CBD and CBN in Cannabis plants.
