Identification of Vaginal Fluid Using Microbial Signatures.
10.3969/j.issn.1004-5619.2016.04.004
- Author:
Kai Nan ZOU
1
;
Meng HU
2
;
Jiang Ping HUANG
1
;
Huai Gu ZHOU
1
Author Information
1. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
2. Railway Police College, Zhengzhou 450053, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
genetic markers;
microbiology;
vaginal fluid
- MeSH:
Actinobacteria/classification*;
Blood/microbiology*;
Body Fluids/microbiology*;
Feces/microbiology*;
Female;
Genes, Bacterial;
Humans;
Lactobacillus/classification*;
Nasal Cavity/microbiology*;
Polymerase Chain Reaction;
RNA, Ribosomal, 16S/genetics*;
Saliva/microbiology*;
Semen/microbiology*;
Vagina/microbiology*
- From:
Journal of Forensic Medicine
2016;32(4):254-256
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the specific microbial signatures in vaginal fluid.
METHODS:Vaginal fluid （16 samples）, saliva （16 samples）, feces （16 samples）, semen （8 samples）, peripheral blood （8 samples）, urine （5 samples）, and nasal secretion （4 samples） were collected respectively. The 16S rRNA genes of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae were amplified. PCR production was detected via a 3130xl Genetic Analyzer.
RESULTS:The detected number of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae were 15, 5, 8, 14, and 3 in all vaginal fluid samples, respectively. Lactobacillus crispatus and Lactobacillus jensenii existed specifically in vaginal fluid.
CONCLUSIONS:There is a potential application value to detect Lactobacillus crispatus and Lactobacillus jensenii for the identification of vaginal fluid.
