Analysis of Differentially Expressed Proteins Distribution in the Rat Brains with DAI by MALDI-TOF-IMS.
10.3969/j.issn.1004-5619.2016.04.001
- Author:
Guan Heng REN
1
;
Rong Hua WENG
2
;
Yan SHI
1
;
Ping HUANG
1
;
Kai Fei DENG
1
;
Ning Guo LIU
1
;
Yi Jiu CHEN
1
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China.
2. Chengxiang Branch of Putian Public Security Bureau, Putian 351100, China.
- Publication Type:Journal Article
- Keywords:
brain stem;
diffuse axonal injury;
forensic pathology;
mass spectrometry;
matrix-assisted laser desorptionionization;
rats
- MeSH:
Animals;
Brain/pathology*;
Diffuse Axonal Injury/pathology*;
Proteins/metabolism*;
Proteome/metabolism*;
Proteomics;
Rats;
Software;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From:
Journal of Forensic Medicine
2016;32(4):241-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To establish the imaging mass spectrometry for analysis of differentially expressed proteins distribution in the rat brains with diffuse axonal injury （DAI） based on matrix assisted laser desorption/ionization-time of flight imaging mass spectrometry （MALDI-TOF-IMS）.
METHODS:MALDI-TOF-IMS scanning were conducted on the brains of DAI group and control group in the m/z range of 1 000 to 20 000 using AutoflexⅢ MALDI-TOF spectrometer. ClinProTool 2.2 software was used for statistical analysis on the data of two groups, and then the differentially expressed proteins were picked out to conduct imaging. The distribution of the proteins with different m/z in the rat brains was observed.
RESULTS:Five proteins with different m/z, including 4 963, 5 634, 6 253, 6 714 and 7 532, differentially expressed in the rat brains with DAI.
CONCLUSIONS:MALDI-TOF-IMS can be used for studying the differentially expressed proteins in rat brains with DAI and the analysis method is established for exploring the distribution of differentially expressed proteins in the rat brains with DAI using imaging mass spectrometry.
