Recognition of Y Fragment Deletion by Genotyping Graphs after Amplified by PowerPlex® 21 Detection Kit.
10.3969/j.issn.1004-5619.2016.03.008
- Author:
San Cun WANG
1
;
Mei Man DING
1
;
Xiao Lin WEI
1
;
Tao ZHANG
2
;
Fei YAO
1
Author Information
1. DNA Lab of Criminal Investigation Detachment, Jiaxing Public Security Bureau, Jiaxing 314001, China.
2. Criminal Investigation Team, Nanhu District Branch Bureau, Jiaxing Public Security Bureau, Jiaxing 314000, China.
- Publication Type:Journal Article
- Keywords:
Amelogenin gene;
DNA fingerprinting;
forensic genetics;
gene frequency
- MeSH:
Alleles;
Amelogenin/genetics*;
Asian People/genetics*;
DNA Fingerprinting/methods*;
Female;
Genotype;
Humans;
Male;
Mutation;
Polymerase Chain Reaction/methods*;
Population Groups
- From:
Journal of Forensic Medicine
2016;32(3):193-195
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To recognize the possibility of Y fragment deletion of Amelogenin gene intuitively and simply according to the genotyping graphs.
METHODS:By calculating the ratio of total peak height of genotyping graphs, the statistics of equilibrium distribution between Amelogenin and D3S1358 loci, Amelogenin X-gene and Amelogenin Y-gene, and different alleles of D3S1358 loci from 1 968 individuals was analyzed after amplified by PowerPlex® 21 detection kit.
RESULTS:Sum of peak height of Amelogenin X allele was not less than 60% that of D3S1358 loci alleles in 90.8% female samples, and sum of peak height of Amelogenin X allele was not higher than 70% that of D3S1358 loci alleles in 94.9% male samples.
CONCLUSIONS:The result of genotyping after amplified by PowerPlex® 21 detection kit shows that the possibility of Y fragment deletion should be considered when only Amelogenin X-gene of Amelogenin is detected and the peak height of Amelogenin X-gene is not higher than 70% of the total peak height of D3S1358 loci.
