Expressions of Mast Cell Tryptase and Brain Natriuretic Peptide in Myocardium of Sudden Death due to Hypersensitivity and Coronary Atherosclerotic Heart Disease.
10.3969/j.issn.1004-5619.2016.03.001
- Author:
Jie Ru SHI
1
;
Cheng Jun TIAN
2
;
Qiang ZENG
1
;
Xiang Jie GUO
1
;
Jian LU
1
;
Cai Rong GAO
1
Author Information
1. Department of Forensic Pathology, School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
2. Public Security Bureau of Yu Country, Yangquan 045100, China.
- Publication Type:Journal Article
- Keywords:
anaphylaxis;
coronary arteriosclerosis;
death, sudden;
forensic pathology;
mast cells;
natriuretic peptide, brain;
tryptases
- MeSH:
Anaphylaxis;
Autopsy;
Blotting, Western;
Case-Control Studies;
Coronary Artery Disease/complications*;
Death, Sudden, Cardiac/etiology*;
Diagnosis, Differential;
Fluorescent Antibody Technique;
Forensic Pathology;
Humans;
Male;
Myocardial Infarction;
Myocardium/metabolism*;
Natriuretic Peptide, Brain/metabolism*;
Tryptases/metabolism*
- From:
Journal of Forensic Medicine
2016;32(3):161-164
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To explore the value of mast cell tryptase and brain natriuretic peptide（BNP） in the differential diagnostic of sudden death due to hypersensitivity and coronary atherosclerotic heart disease.
METHODS:Totally 30 myocardial samples were collected from the autopsy cases in the Department of Forensic Pathology, Shanxi Medical University during 2010-2015. All samples were divided into three groups： death of craniocerebral injury group, sudden death of hypersensitivity group and sudden death of coronary atherosclerotic heart disease group, 10 cases in each group. Mast cell tryptase and BNP in myocardium were detected by immunofluorescence staining and Western Blotting.
RESULTS:Immunofluorescence staining showed that the positive staining mast cell tryptase appeared in myocardium of sudden death of hypersensitivity group and coronary atherosclerotic heart disease group. Among the three groups, the expression of mast cell tryptase showed significantly differences through pairwise comparison （P<0.05）; The expression level of BNP in sudden death of coronary atherosclerotic heart disease group were significantly higher than the sudden death of hypersensitivity group and death of craniocerebral injury group （P<0.05）. The difference of the expression level of BNP between the sudden death of hypersensitivity group and the death of craniocerebral injury group had no statistical significance （P>0.05）.
CONCLUSIONS:The combined detection of the mast cell tryptase and BNP in myocardium is expected to provide help for the forensic differential diagnosis of sudden death due to hypersensitivity and coronary atherosclerotic heart disease.
